Gene description for Nasp
Gene name nuclear autoantigenic sperm protein (histone-binding)
Gene symbol Nasp
Other names/aliases 5033430J04Rik
AI131596
AI317140
D4Ertd767e
Epcs32
Nasp-T
Species Mus musculus
 Database cross references - Nasp
ExoCarta ExoCarta_50927
Vesiclepedia VP_50927
Entrez Gene 50927
UniProt Q99MD9  
 Nasp identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Nasp
Molecular Function
    protein binding GO:0005515 IPI
    histone binding GO:0042393 IBA
    histone binding GO:0042393 IDA
    histone binding GO:0042393 ISO
    protein-containing complex binding GO:0044877 ISO
    histone chaperone activity GO:0140713 IDA
Biological Process
    G1/S transition of mitotic cell cycle GO:0000082 IDA
    blastocyst development GO:0001824 IMP
    DNA replication GO:0006260 IEA
    nucleosome assembly GO:0006334 ISO
    DNA replication-dependent chromatin assembly GO:0006335 IBA
    DNA replication-dependent chromatin assembly GO:0006335 IDA
    DNA replication-dependent chromatin assembly GO:0006335 IDA
    protein transport GO:0015031 IEA
    CENP-A containing chromatin assembly GO:0034080 IBA
Subcellular Localization
    chromatin GO:0000785 ISO
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IDA
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Nasp in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nasp
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hspa2 15512
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
Co-purification Mus musculus
Affinity Capture-Western Mus musculus
2 Tfe3  
Affinity Capture-MS Mus musculus
3 Cdk1 12534
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
4 Atxn1  
Proximity Label-MS Mus musculus
5 Snx1  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Nasp is involved
No pathways found





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