Gene description for Nasp
Gene name
nuclear autoantigenic sperm protein (histone-binding)
Gene symbol
Nasp
Other names/aliases
5033430J04Rik AI131596 AI317140 D4Ertd767e Epcs32 Nasp-T
Species
Mus musculus
Database cross references - Nasp
ExoCarta
ExoCarta_50927
Vesiclepedia
VP_50927
Entrez Gene
50927
UniProt
Q99MD9
Nasp identified in sEVs derived from the following tissue/cell type
Colon cancer cells
37309723
T-cell lymphoma cells
37309723
Gene ontology annotations for Nasp
Experiment description of studies that identified Nasp in sEVs
1
Experiment ID
907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
37309723
Organism
Mus musculus
Experiment description
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name
Proteomics
Publication year
2023
Sample
Colon cancer cells
Sample name
C26
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
2
Experiment ID
908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
37309723
Organism
Mus musculus
Experiment description
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name
Proteomics
Publication year
2023
Sample
T-cell lymphoma cells
Sample name
EL4
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
Protein-protein interactions for Nasp
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
Hspa2
15512
Reconstituted Complex
Mus musculus
Reconstituted Complex
Mus musculus
Co-purification
Mus musculus
Affinity Capture-Western
Mus musculus
2
Tfe3
Affinity Capture-MS
Mus musculus
3
Cdk1
12534
Affinity Capture-Western
Mus musculus
Reconstituted Complex
Mus musculus
Affinity Capture-Western
Mus musculus
4
Atxn1
Proximity Label-MS
Mus musculus
5
Snx1
Co-fractionation
Mus musculus
View the network
image/svg+xml
Pathways in which Nasp is involved
No pathways found