Gene description for TMTC3
Gene name transmembrane and tetratricopeptide repeat containing 3
Gene symbol TMTC3
Other names/aliases SMILE
Species Homo sapiens
 Database cross references - TMTC3
ExoCarta ExoCarta_160418
Vesiclepedia VP_160418
Entrez Gene 160418
HGNC 26899
UniProt Q6ZXV5  
 TMTC3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TMTC3
Molecular Function
    mannosyltransferase activity GO:0000030 IBA
    dolichyl-phosphate-mannose-protein mannosyltransferase activity GO:0004169 IMP
    protein binding GO:0005515 IPI
Biological Process
    response to endoplasmic reticulum stress GO:0034976 IMP
    protein O-linked mannosylation GO:0035269 IBA
    protein O-linked mannosylation GO:0035269 IMP
    positive regulation of proteasomal protein catabolic process GO:1901800 IMP
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    membrane GO:0016020 IEA
 Experiment description of studies that identified TMTC3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TMTC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NRG1 3084
Affinity Capture-MS Homo sapiens
2 KLRC4  
Affinity Capture-MS Homo sapiens
3 PCDHB1  
Affinity Capture-MS Homo sapiens
4 HTR3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 LYZL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 LAMC3  
Affinity Capture-MS Homo sapiens
7 PSCA 8000
Affinity Capture-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 TRHDE 29953
Affinity Capture-MS Homo sapiens
10 SPRTN  
Affinity Capture-MS Homo sapiens
11 CEACAM8 1088
Affinity Capture-MS Homo sapiens
12 PCDHB7  
Affinity Capture-MS Homo sapiens
13 CHRNA4  
Affinity Capture-MS Homo sapiens
14 TTYH1  
Affinity Capture-MS Homo sapiens
15 GABRE  
Affinity Capture-MS Homo sapiens
16 SRRM1 10250
Cross-Linking-MS (XL-MS) Homo sapiens
17 TP53 7157
Affinity Capture-MS Homo sapiens
18 NR3C1 2908
Proximity Label-MS Homo sapiens
19 SEC61B 10952
Proximity Label-MS Homo sapiens
20 PDIA4 9601
Proximity Label-MS Homo sapiens
21 PXMP2  
Proximity Label-MS Homo sapiens
22 DNASE2B  
Affinity Capture-MS Homo sapiens
23 ENPP6 133121
Affinity Capture-MS Homo sapiens
24 GGH 8836
Affinity Capture-MS Homo sapiens
25 CFC1  
Affinity Capture-MS Homo sapiens
26 HTR3C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 NOP56 10528
Proximity Label-MS Homo sapiens
28 CRELD1 78987
Affinity Capture-MS Homo sapiens
29 SYNE4  
Affinity Capture-MS Homo sapiens
30 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
31 PARK2  
Affinity Capture-MS Homo sapiens
32 FBXO6 26270
Affinity Capture-MS Homo sapiens
33 PLD4  
Affinity Capture-MS Homo sapiens
34 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
35 LMNA 4000
Proximity Label-MS Homo sapiens
36 HSPA5 3309
Proximity Label-MS Homo sapiens
37 RPA3 6119
Proximity Label-MS Homo sapiens
38 CHRNB2  
Affinity Capture-MS Homo sapiens
39 CALU 813
Proximity Label-MS Homo sapiens
40 CHRNB4  
Affinity Capture-MS Homo sapiens
41 B3GNT2 10678
Affinity Capture-MS Homo sapiens
42 CDH5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 MB21D1  
Proximity Label-MS Homo sapiens
44 FBXO2 26232
Affinity Capture-MS Homo sapiens
45 DEFA5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 NCEH1 57552
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 LAMB3 3914
Affinity Capture-MS Homo sapiens
48 DNAJC10 54431
Proximity Label-MS Homo sapiens
49 DNAJC3 5611
Proximity Label-MS Homo sapiens
50 DNAJB1 3337
Affinity Capture-MS Homo sapiens
51 KIR2DS3  
Affinity Capture-MS Homo sapiens
52 PLD3 23646
Affinity Capture-MS Homo sapiens
53 DNAJC30  
Proximity Label-MS Homo sapiens
54 LRRC59 55379
Proximity Label-MS Homo sapiens
55 CANX 821
Affinity Capture-MS Homo sapiens
56 SDF2L1 23753
Affinity Capture-MS Homo sapiens
57 SCN3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 COTL1 23406
Co-fractionation Homo sapiens
59 AKAP1 8165
Proximity Label-MS Homo sapiens
60 CALR3  
Proximity Label-MS Homo sapiens
61 DAB1 1600
Two-hybrid Homo sapiens
62 DNAJB11 51726
Proximity Label-MS Homo sapiens
63 ZACN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 CHRND  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 CCDC47 57003
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TMTC3 is involved
No pathways found





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