Gene description for SPATA5
Gene name spermatogenesis associated 5
Gene symbol SPATA5
Other names/aliases AFG2
SPAF
Species Homo sapiens
 Database cross references - SPATA5
ExoCarta ExoCarta_166378
Vesiclepedia VP_166378
Entrez Gene 166378
HGNC 18119
MIM 613940
UniProt Q8NB90  
 SPATA5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for SPATA5
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IBA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    preribosome binding GO:1990275 IDA
Biological Process
    chromatin remodeling GO:0006338 IEA
    spermatogenesis GO:0007283 IEA
    brain development GO:0007420 IMP
    cell differentiation GO:0030154 IEA
    ribosomal large subunit biogenesis GO:0042273 IBA
    ribosomal large subunit biogenesis GO:0042273 IDA
    chromatin looping GO:0140588 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IDA
    spindle GO:0005819 IDA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified SPATA5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SPATA5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 WBSCR16  
Affinity Capture-MS Homo sapiens
2 KBTBD4  
Affinity Capture-MS Homo sapiens
3 Cbx1  
Affinity Capture-MS Mus musculus
4 RIT1 6016
Negative Genetic Homo sapiens
5 SPATA5L1 79029
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 FBXW11  
Affinity Capture-MS Homo sapiens
8 MAPRE1 22919
Affinity Capture-MS Homo sapiens
9 SIRT6  
Affinity Capture-MS Homo sapiens
10 TIMD4  
Affinity Capture-MS Homo sapiens
11 AIFM1 9131
Affinity Capture-MS Homo sapiens
12 Psmb4 19172
Affinity Capture-MS Mus musculus
13 RPL13 6137
Affinity Capture-MS Homo sapiens
14 AMBRA1  
Affinity Capture-MS Homo sapiens
15 FAS 355
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CINP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 DNASE2B  
Affinity Capture-MS Homo sapiens
18 XPO1 7514
Affinity Capture-MS Homo sapiens
19 UFL1 23376
Affinity Capture-MS Homo sapiens
20 TRIM66  
Affinity Capture-MS Homo sapiens
21 B3GNT3 10331
Affinity Capture-MS Homo sapiens
22 USP36  
Affinity Capture-MS Homo sapiens
23 CUL3 8452
Affinity Capture-MS Homo sapiens
24 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
25 EGFR 1956
Negative Genetic Homo sapiens
26 PARK2  
Affinity Capture-MS Homo sapiens
27 C1orf109  
Affinity Capture-MS Homo sapiens
28 RIPK4  
Affinity Capture-MS Homo sapiens
29 SSR2  
Affinity Capture-MS Homo sapiens
30 PSMD14 10213
Affinity Capture-MS Homo sapiens
31 ATAD3A 55210
Affinity Capture-MS Homo sapiens
32 AURKB 9212
Affinity Capture-MS Homo sapiens
33 TMEM25  
Affinity Capture-MS Homo sapiens
34 C9orf78 51759
Affinity Capture-MS Homo sapiens
35 PFDN5 5204
Affinity Capture-MS Homo sapiens
36 S100A4 6275
Affinity Capture-MS Homo sapiens
37 PFDN2 5202
Affinity Capture-MS Homo sapiens
38 PSMA5 5686
Affinity Capture-MS Homo sapiens
39 DYRK1A 1859
Affinity Capture-MS Homo sapiens
40 HECTD1 25831
Affinity Capture-MS Homo sapiens
41 NTRK1 4914
Affinity Capture-MS Homo sapiens
42 GPR45  
Affinity Capture-MS Homo sapiens
43 IFI16 3428
Affinity Capture-MS Homo sapiens
44 Rrbp1  
Affinity Capture-MS Mus musculus
45 FAM174A 345757
Affinity Capture-MS Homo sapiens
46 C15orf26  
Affinity Capture-MS Homo sapiens
47 BRD1 23774
Affinity Capture-MS Homo sapiens
48 PSMC5 5705
Affinity Capture-MS Homo sapiens
49 SPATA5 166378
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 SNW1 22938
Affinity Capture-MS Homo sapiens
51 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
52 RCN2 5955
Affinity Capture-MS Homo sapiens
53 P2RY10  
Affinity Capture-MS Homo sapiens
54 DDRGK1 65992
Affinity Capture-MS Homo sapiens
55 MYC  
Affinity Capture-MS Homo sapiens
56 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 PSMC4 5704
Affinity Capture-MS Homo sapiens
58 MEX3A  
Affinity Capture-RNA Homo sapiens
59 SP100 6672
Affinity Capture-MS Homo sapiens
60 C9orf72  
Affinity Capture-MS Homo sapiens
61 RPL15 6138
Affinity Capture-MS Homo sapiens
62 CDC5L 988
Affinity Capture-MS Homo sapiens
63 DKKL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 RAN 5901
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SPATA5 is involved
No pathways found





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