Gene description for Lgals1
Gene name lectin, galactose binding, soluble 1
Gene symbol Lgals1
Other names/aliases AA410090
Gal-1
Galbp
L-14.5
L14
Lect14
galectin-1
Species Mus musculus
 Database cross references - Lgals1
ExoCarta ExoCarta_16852
Vesiclepedia VP_16852
Entrez Gene 16852
UniProt P16045  
 Lgals1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Neural stem cells 25242146    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Lgals1
Molecular Function
    protein binding GO:0005515 IPI
    galactose binding GO:0005534 NAS
    carbohydrate binding GO:0030246 IDA
    lactose binding GO:0030395 IBA
    lactose binding GO:0030395 ISO
    identical protein binding GO:0042802 ISO
    laminin binding GO:0043236 IBA
    laminin binding GO:0043236 ISO
    receptor ligand activity GO:0048018 IEA
Biological Process
    plasma cell differentiation GO:0002317 IDA
    apoptotic process GO:0006915 IEA
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 NAS
    signal transduction GO:0007165 IEA
    negative regulation of cell-substrate adhesion GO:0010812 ISO
    negative regulation of neuron projection development GO:0010977 ISO
    T cell costimulation GO:0031295 IDA
    positive regulation of erythrocyte aggregation GO:0034120 ISO
    positive regulation of apoptotic process GO:0043065 ISO
    maintenance of protein location GO:0045185 IDA
    myoblast differentiation GO:0045445 IDA
    positive regulation of viral entry into host cell GO:0046598 IEA
    positive regulation of viral entry into host cell GO:0046598 ISO
    positive regulation of inflammatory response GO:0050729 ISO
    cell-cell adhesion GO:0098609 ISO
    negative regulation of T-helper 17 cell lineage commitment GO:2000329 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    cell surface GO:0009986 ISO
    collagen-containing extracellular matrix GO:0062023 HDA
    galectin complex GO:1990724 ISO
 Experiment description of studies that identified Lgals1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Lgals1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Psmg1 56088
Co-fractionation Mus musculus
2 Atxn1  
Proximity Label-MS Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Snrpd2 107686
Co-fractionation Mus musculus
5 Fubp1  
Co-fractionation Mus musculus
6 Vcl 22330
Co-fractionation Mus musculus
7 Fancd2  
Affinity Capture-MS Mus musculus
8 Snrpd3 67332
Co-fractionation Mus musculus
9 Kcnma1  
Affinity Capture-MS Mus musculus
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