Gene description for Vcl
Gene name vinculin
Gene symbol Vcl
Other names/aliases 9430097D22
AA571387
AI462105
AW545629
Species Mus musculus
 Database cross references - Vcl
ExoCarta ExoCarta_22330
Vesiclepedia VP_22330
Entrez Gene 22330
UniProt Q64727  
 Vcl identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Vcl
Molecular Function
    dystroglycan binding GO:0002162 IEA
    dystroglycan binding GO:0002162 ISO
    actin binding GO:0003779 ISO
    structural molecule activity GO:0005198 IEA
    protein binding GO:0005515 IPI
    beta-catenin binding GO:0008013 IBA
    small GTPase binding GO:0031267 ISO
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    alpha-catenin binding GO:0045294 IBA
    alpha-catenin binding GO:0045294 IEA
    alpha-catenin binding GO:0045294 ISO
    actin filament binding GO:0051015 IEA
    molecular adaptor activity GO:0060090 ISO
Biological Process
    morphogenesis of an epithelium GO:0002009 IEA
    morphogenesis of an epithelium GO:0002009 ISO
    cell adhesion GO:0007155 IBA
    cell adhesion GO:0007155 IDA
    lamellipodium assembly GO:0030032 IDA
    regulation of cell migration GO:0030334 IDA
    adherens junction assembly GO:0034333 IEA
    adherens junction assembly GO:0034333 ISO
    protein localization to cell surface GO:0034394 IEA
    protein localization to cell surface GO:0034394 ISO
    apical junction assembly GO:0043297 IEA
    apical junction assembly GO:0043297 ISO
    axon extension GO:0048675 IGI
    regulation of focal adhesion assembly GO:0051893 IEA
    regulation of focal adhesion assembly GO:0051893 ISO
    epithelial cell-cell adhesion GO:0090136 IEA
    epithelial cell-cell adhesion GO:0090136 ISO
    regulation of establishment of endothelial barrier GO:1903140 IEA
    regulation of establishment of endothelial barrier GO:1903140 ISO
    regulation of protein localization to adherens junction GO:1904702 IEA
    regulation of protein localization to adherens junction GO:1904702 ISO
Subcellular Localization
    stress fiber GO:0001725 ISO
    podosome GO:0002102 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoskeleton GO:0005856 IBA
    actin filament GO:0005884 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    brush border GO:0005903 ISS
    cell-cell junction GO:0005911 IDA
    cell-cell junction GO:0005911 ISO
    adherens junction GO:0005912 IBA
    adherens junction GO:0005912 IDA
    adherens junction GO:0005912 ISO
    zonula adherens GO:0005915 ISS
    fascia adherens GO:0005916 IDA
    focal adhesion GO:0005925 IDA
    focal adhesion GO:0005925 ISO
    intercalated disc GO:0014704 ISO
    actin cytoskeleton GO:0015629 IDA
    Z disc GO:0030018 ISO
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
    sarcolemma GO:0042383 IDA
    sarcolemma GO:0042383 ISO
    cell projection GO:0042995 IEA
    costamere GO:0043034 IDA
    costamere GO:0043034 ISO
    cell-cell contact zone GO:0044291 IBA
    cell-cell contact zone GO:0044291 ISO
    membrane raft GO:0045121 ISO
    podosome ring GO:0061826 IDA
    outer dense plaque of desmosome GO:0090636 ISS
    inner dense plaque of desmosome GO:0090637 ISS
    terminal web GO:1990357 ISS
 Experiment description of studies that identified Vcl in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Vcl
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Raver1  
Two-hybrid Mus musculus
Far Western Mus musculus
2 Ctnnb1 12387
Co-fractionation Mus musculus
3 Hspb2 69253
Affinity Capture-MS Mus musculus
4 Msn 17698
Co-fractionation Mus musculus
5 Sorbs2  
Reconstituted Complex Mus musculus
6 Myh6  
Cross-Linking-MS (XL-MS) Mus musculus
7 Kctd13  
Affinity Capture-MS Mus musculus
8 Asap1  
Co-fractionation Mus musculus
9 Myl6 17904
Co-fractionation Mus musculus
10 Fermt3 108101
Co-fractionation Mus musculus
11 Ptk2  
Co-fractionation Mus musculus
12 Tpm4  
Co-fractionation Mus musculus
13 Fzr1  
Affinity Capture-MS Mus musculus
14 Immt  
Cross-Linking-MS (XL-MS) Mus musculus
15 Tln1 21894
Co-fractionation Mus musculus
16 Unk  
Affinity Capture-RNA Mus musculus
17 HACE1 57531
Affinity Capture-MS Homo sapiens
18 Arhgef2  
Co-fractionation Mus musculus
19 Dag1 13138
Co-fractionation Mus musculus
20 Pfas 237823
Co-fractionation Mus musculus
21 Pdlim5 56376
Affinity Capture-Western Mus musculus
22 Ubfd1  
Co-fractionation Mus musculus
23 Lgals1 16852
Co-fractionation Mus musculus
24 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here