Gene description for Pfas
Gene name phosphoribosylformylglycinamidine synthase (FGAR amidotransferase)
Gene symbol Pfas
Other names/aliases 4432409B16Rik
FGAMS
FGAR-AT
FGARAT
Gm18
PURL
Sofa
Species Mus musculus
 Database cross references - Pfas
ExoCarta ExoCarta_237823
Vesiclepedia VP_237823
Entrez Gene 237823
UniProt Q5SUR0  
 Pfas identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pfas
Molecular Function
    phosphoribosylformylglycinamidine synthase activity GO:0004642 IBA
    phosphoribosylformylglycinamidine synthase activity GO:0004642 ISO
    ATP binding GO:0005524 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    purine nucleotide biosynthetic process GO:0006164 IBA
    GMP biosynthetic process GO:0006177 ISO
    'de novo' IMP biosynthetic process GO:0006189 IEA
    'de novo' IMP biosynthetic process GO:0006189 ISO
    glutamine metabolic process GO:0006541 IEA
    glutamine metabolic process GO:0006541 ISO
    response to xenobiotic stimulus GO:0009410 IEA
    'de novo' AMP biosynthetic process GO:0044208 ISO
    anterior head development GO:0097065 IMP
    'de novo' XMP biosynthetic process GO:0097294 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified Pfas in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pfas
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atxn1  
Affinity Capture-MS Mus musculus
2 Vcl 22330
Co-fractionation Mus musculus
3 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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