Gene description for Myl6
Gene name myosin, light polypeptide 6, alkali, smooth muscle and non-muscle
Gene symbol Myl6
Other names/aliases ESMLC
LC17
LC17-GI
LC17-NM
LC17A
LC17B
MLC-3
MLC1SM
MLC3SM
MLC3nm
Myln
Species Mus musculus
 Database cross references - Myl6
ExoCarta ExoCarta_17904
Vesiclepedia VP_17904
Entrez Gene 17904
UniProt Q60605  
 Myl6 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Microglia 16081791    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Myl6
Molecular Function
    microfilament motor activity GO:0000146 ISS
    cytoskeletal motor activity GO:0003774 TAS
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    structural constituent of muscle GO:0008307 IBA
    structural constituent of muscle GO:0008307 ISO
    structural constituent of muscle GO:0008307 ISS
    structural constituent of muscle GO:0008307 TAS
Biological Process
    muscle contraction GO:0006936 TAS
    skeletal muscle tissue development GO:0007519 TAS
    muscle filament sliding GO:0030049 TAS
Subcellular Localization
    brush border GO:0005903 ISO
    myosin complex GO:0016459 TAS
    myosin II complex GO:0016460 IBA
    unconventional myosin complex GO:0016461 IDA
 Experiment description of studies that identified Myl6 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Myl6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Twist1  
Proximity Label-MS Mus musculus
2 Lmo2  
Two-hybrid Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Nlrp3  
Affinity Capture-MS Mus musculus
5 Cebpa  
Affinity Capture-MS Mus musculus
6 Vcl 22330
Co-fractionation Mus musculus
7 Fancd2  
Affinity Capture-MS Mus musculus
8 Kctd13  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here