Gene description for Mcm6
Gene name minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae)
Gene symbol Mcm6
Other names/aliases ASP-l1
D1Wsu22e
Mcmd6
Species Mus musculus
 Database cross references - Mcm6
ExoCarta ExoCarta_17219
Vesiclepedia VP_17219
Entrez Gene 17219
UniProt P97311  
 Mcm6 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mcm6
Molecular Function
    DNA helicase activity GO:0003678 IDA
    DNA helicase activity GO:0003678 IMP
    DNA helicase activity GO:0003678 ISO
    DNA helicase activity GO:0003678 ISS
    DNA clamp loader activity GO:0003689 IEA
    single-stranded DNA binding GO:0003697 IDA
    single-stranded DNA binding GO:0003697 IMP
    single-stranded DNA binding GO:0003697 IPI
    single-stranded DNA binding GO:0003697 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    four-way junction helicase activity GO:0009378 IEA
    ATP hydrolysis activity GO:0016887 IEA
    double-stranded DNA helicase activity GO:0036121 IEA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IBA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    double-strand break repair via break-induced replication GO:0000727 IBA
    DNA unwinding involved in DNA replication GO:0006268 IBA
    DNA unwinding involved in DNA replication GO:0006268 IPI
    DNA unwinding involved in DNA replication GO:0006268 ISO
    DNA replication initiation GO:0006270 IEA
    premeiotic DNA replication GO:0006279 NAS
    chromatin remodeling GO:0006338 IEA
    chromatin looping GO:0140588 IEA
    mitotic DNA replication GO:1902969 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 ISO
    MCM complex GO:0042555 IBA
    MCM complex GO:0042555 IDA
    MCM complex GO:0042555 ISO
    MCM complex GO:0042555 ISS
    CMG complex GO:0071162 ISO
    CMG complex GO:0071162 ISS
 Experiment description of studies that identified Mcm6 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mcm6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mdm2  
Affinity Capture-MS Mus musculus
2 MCM10 55388
Two-hybrid Homo sapiens
3 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
4 Lmo2  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Atrx  
Affinity Capture-Western Mus musculus
Co-localization Mus musculus
7 CDC7  
Biochemical Activity Homo sapiens
8 Dmrtc2  
Two-hybrid Mus musculus
9 Chaf1a  
Affinity Capture-MS Mus musculus
10 Gmeb2  
Two-hybrid Mus musculus
11 Fancd2  
Affinity Capture-MS Mus musculus
12 ATRX 546
Affinity Capture-Western Homo sapiens
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