Gene description for DHODH
Gene name dihydroorotate dehydrogenase (quinone)
Gene symbol DHODH
Other names/aliases DHOdehase
POADS
URA1
Species Homo sapiens
 Database cross references - DHODH
ExoCarta ExoCarta_1723
Vesiclepedia VP_1723
Entrez Gene 1723
HGNC 2867
MIM 126064
UniProt Q02127  
 DHODH identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for DHODH
Molecular Function
    dihydroorotase activity GO:0004151 IEA
    dihydroorotate dehydrogenase activity GO:0004152 IBA
    protein binding GO:0005515 IPI
    dihydroorotate dehydrogenase (quinone) activity GO:0106430 IDA
Biological Process
    'de novo' pyrimidine nucleobase biosynthetic process GO:0006207 IBA
    UDP biosynthetic process GO:0006225 IEA
    pyrimidine ribonucleotide biosynthetic process GO:0009220 IBA
    'de novo' UMP biosynthetic process GO:0044205 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IBA
    mitochondrial inner membrane GO:0005743 TAS
    cytosol GO:0005829 IDA
 Experiment description of studies that identified DHODH in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DHODH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SDHD  
Affinity Capture-MS Homo sapiens
2 FTL 2512
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 SDHC 6391
Affinity Capture-MS Homo sapiens
5 TMED10 10972
Two-hybrid Homo sapiens
6 GMPPB 29925
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 ILF3 3609
Co-fractionation Homo sapiens
8 SDHA 6389
Co-fractionation Homo sapiens
9 VAC14 55697
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 RNF19B  
Two-hybrid Homo sapiens
11 TMEM95  
Affinity Capture-MS Homo sapiens
12 HNRNPM 4670
Co-fractionation Homo sapiens
13 FKBP8 23770
Co-fractionation Homo sapiens
14 VHL  
Negative Genetic Homo sapiens
15 BAP1 8314
Negative Genetic Homo sapiens
16 SSBP1 6742
Co-fractionation Homo sapiens
17 MT2A 4502
Two-hybrid Homo sapiens
18 ACACA 31
Negative Genetic Homo sapiens
19 NDUFS8 4728
Co-fractionation Homo sapiens
20 OGDH 4967
Co-fractionation Homo sapiens
21 BST1 683
Affinity Capture-MS Homo sapiens
22 C1orf54  
Affinity Capture-MS Homo sapiens
23 SERBP1 26135
Affinity Capture-MS Homo sapiens
24 CYC1 1537
Co-fractionation Homo sapiens
25 KRAS 3845
Negative Genetic Homo sapiens
26 PLP2 5355
Co-fractionation Homo sapiens
27 TTPA  
Two-hybrid Homo sapiens
28 SLC31A1 1317
Affinity Capture-MS Homo sapiens
29 PIPSL 266971
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DHODH is involved
PathwayEvidenceSource
Metabolism TAS Reactome
Metabolism of nucleotides TAS Reactome
Nucleotide biosynthesis TAS Reactome
Pyrimidine biosynthesis TAS Reactome





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