Gene description for MT2A
Gene name metallothionein 2A
Gene symbol MT2A
Other names/aliases MT2
Species Homo sapiens
 Database cross references - MT2A
ExoCarta ExoCarta_4502
Vesiclepedia VP_4502
Entrez Gene 4502
HGNC 7406
MIM 156360
UniProt P02795  
 MT2A identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for MT2A
Molecular Function
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 ISS
    metal ion binding GO:0046872 IBA
Biological Process
    intracellular copper ion homeostasis GO:0006878 TAS
    intracellular zinc ion homeostasis GO:0006882 IBA
    detoxification of copper ion GO:0010273 IBA
    cellular response to interleukin-3 GO:0036016 IEP
    cellular response to erythropoietin GO:0036018 IEP
    negative regulation of growth GO:0045926 ISS
    cellular response to cadmium ion GO:0071276 IBA
    cellular response to copper ion GO:0071280 IBA
    cellular response to zinc ion GO:0071294 IBA
    cellular response to zinc ion GO:0071294 IEP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified MT2A in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MT2A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DHODH 1723
Two-hybrid Homo sapiens
2 PLS1 5357
Affinity Capture-MS Homo sapiens
3 SHBG 6462
Two-hybrid Homo sapiens
4 JMJD1C 221037
Two-hybrid Homo sapiens
5 PAXIP1  
Two-hybrid Homo sapiens
6 PCDHGA5  
Affinity Capture-MS Homo sapiens
7 RNF181  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
8 ECT2 1894
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 PFN1 5216
Co-fractionation Homo sapiens
10 ASS1 445
Two-hybrid Homo sapiens
11 PRKD1 5587
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
12 RCHY1  
Two-hybrid Homo sapiens
13 ARF6 382
Reconstituted Complex Homo sapiens
14 LAMTOR5 10542
Two-hybrid Homo sapiens
15 TRIM37  
Proximity Label-MS Homo sapiens
16 YAP1 10413
Affinity Capture-MS Homo sapiens
17 BARD1 580
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
18 PPDPF  
Two-hybrid Homo sapiens
19 SPINK7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
20 PTER 9317
Affinity Capture-MS Homo sapiens
21 FUBP1 8880
Co-fractionation Homo sapiens
22 NEDD4L 23327
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
23 MSRA 4482
Co-fractionation Homo sapiens
24 USP25  
Two-hybrid Homo sapiens
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