Experiment description of studies that identified Nras in sEVs

1 Experiment ID 907 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✔ Negative markers ✔ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample Colon cancer cells Sample name C26 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 73 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 18494037     Organism Mus musculus Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes. Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ" Journal name ELEC Publication year 2008 Sample Embryonic fibroblasts Sample name NIH3T3 Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LCQ DECA] Western blotting

3 Experiment ID 210 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23260141     Organism Mus musculus Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration. Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL." Journal name Cell Publication year 2012 Sample Fibroblasts Sample name Normal-Fibroblasts (L cells) Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 15 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 17486113     Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO" Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array

5 Experiment ID 908 MISEV standards ✔ Biophysical techniques ✔ Enriched markers ✔ Negative markers ✔ Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample T-cell lymphoma cells Sample name EL4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Nras

Protein Interactor ExoCarta ID Identification method PubMed Species 1 SMURF2 64750 Affinity Capture-Luminescence Homo sapiens 2 Trim28 21849 Synthetic Lethality Mus musculus 3 Bcl2   Synthetic Lethality Mus musculus 4 Prdm4   Synthetic Lethality Mus musculus 5 SMAD4   Affinity Capture-Luminescence Homo sapiens 6 Shoc2   Synthetic Lethality Mus musculus 7 Urm1   Synthetic Lethality Mus musculus 8 Rce1   Synthetic Lethality Mus musculus 9 Raf1   Synthetic Lethality Mus musculus 10 Rps6ka1 20111 Synthetic Lethality Mus musculus 11 ACVR1 90 Affinity Capture-Luminescence Homo sapiens 12 Icmt   Synthetic Lethality Mus musculus 13 Pa2g4 18813 Synthetic Lethality Mus musculus 14 Braf 109880 Synthetic Lethality Mus musculus 15 Cherp   Synthetic Lethality Mus musculus 16 Ctu1   Synthetic Lethality Mus musculus 17 Mapk1 26413 Synthetic Lethality Mus musculus 18 Traf3   Proximity Label-MS Mus musculus 19 Usp32   Synthetic Lethality Mus musculus 20 Rac1 19353 Synthetic Lethality Mus musculus 21 Golga7 57437 Synthetic Lethality Mus musculus 22 Fancd2   Affinity Capture-MS Mus musculus View the network

Pathways in which Nras is involved

No pathways found