Gene description for P4hb
Gene name prolyl 4-hydroxylase, beta polypeptide
Gene symbol P4hb
Other names/aliases ERp59
PDI
Pdia1
Thbp
Species Mus musculus
 Database cross references - P4hb
ExoCarta ExoCarta_18453
Vesiclepedia VP_18453
Entrez Gene 18453
UniProt P09103  
 P4hb identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for P4hb
Molecular Function
    protein disulfide isomerase activity GO:0003756 IBA
    protein disulfide isomerase activity GO:0003756 IEA
    protein disulfide isomerase activity GO:0003756 ISO
    actin binding GO:0003779 IEA
    actin binding GO:0003779 ISO
    procollagen-proline 4-dioxygenase activity GO:0004656 ISO
    integrin binding GO:0005178 IPI
    integrin binding GO:0005178 ISO
    protein binding GO:0005515 IPI
    protein-disulfide reductase activity GO:0015035 IMP
    protein-disulfide reductase activity GO:0015035 ISO
    thiol oxidase activity GO:0016972 IEA
    thiol oxidase activity GO:0016972 ISO
    enzyme binding GO:0019899 IEA
    enzyme binding GO:0019899 ISO
    protein-containing complex binding GO:0044877 ISO
    protein heterodimerization activity GO:0046982 ISO
    protein heterodimerization activity GO:0046982 ISS
Biological Process
    protein folding GO:0006457 IBA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IMP
    peptidyl-proline hydroxylation to 4-hydroxy-L-proline GO:0018401 ISO
    insulin processing GO:0030070 IMP
    protein folding in endoplasmic reticulum GO:0034975 IEA
    protein folding in endoplasmic reticulum GO:0034975 ISO
    response to endoplasmic reticulum stress GO:0034976 IBA
    response to endoplasmic reticulum stress GO:0034976 IEA
    response to endoplasmic reticulum stress GO:0034976 ISO
    positive regulation of cell adhesion GO:0045785 ISO
    positive regulation of viral entry into host cell GO:0046598 IEA
    positive regulation of viral entry into host cell GO:0046598 ISO
    cellular response to hypoxia GO:0071456 IEA
    cellular response to hypoxia GO:0071456 ISO
    cellular response to interleukin-7 GO:0098761 IDA
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IEA
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 ISO
    regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902175 IEA
    regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902175 ISO
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum lumen GO:0005788 IMP
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IEA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytoskeleton GO:0005856 IEA
    cytoskeleton GO:0005856 ISO
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 IDA
    external side of plasma membrane GO:0009897 ISO
    procollagen-proline 4-dioxygenase complex GO:0016222 ISO
    lamellipodium GO:0030027 IEA
    lamellipodium GO:0030027 ISO
    protein-containing complex GO:0032991 ISO
    endoplasmic reticulum chaperone complex GO:0034663 IDA
    melanosome GO:0042470 IEA
 Experiment description of studies that identified P4hb in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for P4hb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mettl21e  
Affinity Capture-MS Mus musculus
2 Txndc5 105245
Co-fractionation Mus musculus
3 Sdf2l1  
Co-fractionation Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Prnp 19122
Affinity Capture-MS Mus musculus
6 Tpd52l2  
Co-fractionation Mus musculus
7 P4hb 18453
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
8 Rps6 20104
Co-fractionation Mus musculus
9 Fancd2  
Affinity Capture-MS Mus musculus
10 Rrbp1  
Co-fractionation Mus musculus
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