Experiment description of studies that identified Pura in sEVs

1 Experiment ID 907 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample Colon cancer cells Sample name C26 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 908 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample T-cell lymphoma cells Sample name EL4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Pura

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Cdk4   Reconstituted Complex Mus musculus 2 Fxr1 14359 Affinity Capture-MS Mus musculus 3 Tnik   Affinity Capture-MS Mus musculus 4 Cdk1 12534 Reconstituted Complex Mus musculus 5 Pde4dip   Affinity Capture-MS Mus musculus 6 Ccnb1   Reconstituted Complex Mus musculus 7 Ccnd1   Reconstituted Complex Mus musculus 8 Ccnh   Reconstituted Complex Mus musculus 9 Dlgap1   Affinity Capture-MS Mus musculus 10 Cdk2   Co-localization Mus musculus 11 Syngap1   Affinity Capture-MS Mus musculus Affinity Capture-MS Mus musculus 12 Fmr1   Two-hybrid Mus musculus Affinity Capture-MS Mus musculus 13 Kctd13   Affinity Capture-MS Mus musculus 14 Atg16l1   Affinity Capture-MS Mus musculus 15 Huwe1   Affinity Capture-MS Mus musculus 16 Mecp2   Affinity Capture-MS Mus musculus 17 Ywhae 22627 Affinity Capture-MS Mus musculus 18 Ccne1   Reconstituted Complex Mus musculus 19 Fzr1   Affinity Capture-MS Mus musculus 20 Ccna2   Reconstituted Complex Mus musculus 21 E2f1   Affinity Capture-Western Mus musculus Affinity Capture-Western Mus musculus 22 Cdk5 12568 Affinity Capture-Western Mus musculus 23 Purb 19291 Co-fractionation Mus musculus 24 Tsc1   Affinity Capture-MS Mus musculus 25 Hist1h1a   Reconstituted Complex Mus musculus 26 Zbtb7b   Affinity Capture-MS Mus musculus 27 Tia1   Affinity Capture-MS Mus musculus 28 Fancd2   Affinity Capture-MS Mus musculus View the network

Pathways in which Pura is involved

No pathways found