Gene description for Purb
Gene name purine rich element binding protein B
Gene symbol Purb
Other names/aliases 2310015K15Rik
AA114818
Cager-2
D11Bwg0414e
Species Mus musculus
 Database cross references - Purb
ExoCarta ExoCarta_19291
Vesiclepedia VP_19291
Entrez Gene 19291
UniProt O35295  
 Purb identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Purb
Molecular Function
    mRNA regulatory element binding translation repressor activity GO:0000900 ISO
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IBA
    RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000977 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IDA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IMP
    double-stranded DNA binding GO:0003690 IDA
    double-stranded telomeric DNA binding GO:0003691 ISS
    single-stranded DNA binding GO:0003697 IDA
    single-stranded DNA binding GO:0003697 ISO
    DNA-binding transcription factor activity GO:0003700 IDA
    RNA binding GO:0003723 IDA
    mRNA binding GO:0003729 IDA
    protein binding GO:0005515 IPI
    purine-rich negative regulatory element binding GO:0032422 IBA
    purine-rich negative regulatory element binding GO:0032422 IEA
    purine-rich negative regulatory element binding GO:0032422 ISO
    SMAD binding GO:0046332 IDA
    DNA-binding transcription factor binding GO:0140297 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    negative regulation of transcription by RNA polymerase II GO:0000122 IGI
    DNA unwinding involved in DNA replication GO:0006268 ISS
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    negative regulation of DNA-templated transcription GO:0045892 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
 Experiment description of studies that identified Purb in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Purb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fzr1  
Affinity Capture-MS Mus musculus
2 Fmr1  
Affinity Capture-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Pde4dip  
Affinity Capture-MS Mus musculus
5 Mapt  
Affinity Capture-MS Mus musculus
6 Pura 19290
Co-fractionation Mus musculus
7 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Purb is involved
No pathways found





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