Gene description for MEGF8
Gene name multiple EGF-like-domains 8
Gene symbol MEGF8
Other names/aliases C19orf49
CRPT2
EGFL4
SBP1
Species Homo sapiens
 Database cross references - MEGF8
ExoCarta ExoCarta_1954
Vesiclepedia VP_1954
Entrez Gene 1954
HGNC 3233
MIM 604267
UniProt Q7Z7M0  
 MEGF8 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for MEGF8
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
Biological Process
    embryonic heart tube morphogenesis GO:0003143 ISS
    smoothened signaling pathway GO:0007224 IEA
    regulation of gene expression GO:0010468 ISS
    protein ubiquitination GO:0016567 IEA
    embryonic limb morphogenesis GO:0030326 ISS
    BMP signaling pathway GO:0030509 ISS
    limb morphogenesis GO:0035108 IMP
    aorta development GO:0035904 IEA
    cell migration involved in gastrulation GO:0042074 IMP
    embryonic digit morphogenesis GO:0042733 IEA
    negative regulation of smoothened signaling pathway GO:0045879 ISS
    embryonic skeletal system morphogenesis GO:0048704 ISS
    positive regulation of axon extension involved in axon guidance GO:0048842 ISS
    epiboly involved in gastrulation with mouth forming second GO:0055113 IMP
    embryonic heart tube left/right pattern formation GO:0060971 ISS
    left/right pattern formation GO:0060972 IMP
    coronary vasculature development GO:0060976 IEA
    determination of heart left/right asymmetry GO:0061371 IMP
    pharyngeal arch artery morphogenesis GO:0061626 IEA
    protein-containing complex assembly GO:0065003 IEA
    determination of digestive tract left/right asymmetry GO:0071907 ISS
    craniofacial suture morphogenesis GO:0097094 IMP
    fasciculation of sensory neuron axon GO:0097155 ISS
    embryonic brain development GO:1990403 IEA
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IEA
    nucleus GO:0005634 ISS
    membrane GO:0016020 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified MEGF8 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for MEGF8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 ELSPBP1  
Affinity Capture-MS Homo sapiens
3 BTNL2  
Affinity Capture-MS Homo sapiens
4 PSG3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CACNA1A  
Two-hybrid Homo sapiens
6 PSG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 GFI1B  
Two-hybrid Homo sapiens
8 RAB7A 7879
Proximity Label-MS Homo sapiens
9 HLA-G 3135
Affinity Capture-MS Homo sapiens
10 DEFB109P1B  
Affinity Capture-MS Homo sapiens
11 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ALPPL2 251
Affinity Capture-MS Homo sapiens
13 ATXN7  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
14 TRGV3  
Affinity Capture-MS Homo sapiens
15 CUL3 8452
Affinity Capture-MS Homo sapiens
16 NR3C1 2908
Proximity Label-MS Homo sapiens
17 CBLN4  
Affinity Capture-MS Homo sapiens
18 FBXO6 26270
Affinity Capture-MS Homo sapiens
19 SLURP1  
Affinity Capture-MS Homo sapiens
20 ATN1  
Two-hybrid Homo sapiens
21 IL5RA  
Affinity Capture-MS Homo sapiens
22 NXF1 10482
Affinity Capture-RNA Homo sapiens
23 SFTPC  
Affinity Capture-MS Homo sapiens
24 MGRN1 23295
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 MSTN  
Affinity Capture-MS Homo sapiens
26 SIRPD  
Affinity Capture-MS Homo sapiens
27 IGFBP4 3487
Affinity Capture-MS Homo sapiens
28 SDF2L1 23753
Affinity Capture-MS Homo sapiens
29 FIBIN  
Affinity Capture-MS Homo sapiens
30 FBXO2 26232
Affinity Capture-MS Homo sapiens
31 GGH 8836
Affinity Capture-MS Homo sapiens
32 ALPP 250
Affinity Capture-MS Homo sapiens
33 TAGLN2 8407
Cross-Linking-MS (XL-MS) Homo sapiens
34 PTPRK 5796
Affinity Capture-MS Homo sapiens
35 LYPD1  
Affinity Capture-MS Homo sapiens
36 EDN3  
Affinity Capture-MS Homo sapiens
37 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
38 TDGF1  
Affinity Capture-MS Homo sapiens
39 PRELP 5549
Affinity Capture-MS Homo sapiens
40 ANKRD46 157567
Affinity Capture-MS Homo sapiens
41 CFC1  
Affinity Capture-MS Homo sapiens
42 PSG8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 SPCS3 60559
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MEGF8 is involved
No pathways found





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