Gene description for Srsf5
Gene name serine/arginine-rich splicing factor 5
Gene symbol Srsf5
Other names/aliases Sfrs5
Species Mus musculus
 Database cross references - Srsf5
ExoCarta ExoCarta_20384
Vesiclepedia VP_20384
Entrez Gene 20384
UniProt O35326  
 Srsf5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Srsf5
Molecular Function
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IEA
    RNA binding GO:0003723 ISO
    protein binding GO:0005515 IPI
    protein kinase B binding GO:0043422 ISO
    RS domain binding GO:0050733 IPI
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
    response to wounding GO:0009611 IDA
    response to insulin GO:0032868 ISO
    positive regulation of RNA splicing GO:0033120 ISO
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 ISO
    cytosol GO:0005829 ISO
    nuclear speck GO:0016607 IBA
    nuclear speck GO:0016607 IDA
 Experiment description of studies that identified Srsf5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Srsf5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LUC7L2 51631
Two-hybrid Homo sapiens
2 Kctd13  
Affinity Capture-MS Mus musculus
3 PRPF38A 84950
Two-hybrid Homo sapiens
4 Atxn1  
Affinity Capture-MS Mus musculus
5 Cebpa  
Affinity Capture-MS Mus musculus
6 SRSF4 6429
Two-hybrid Homo sapiens
7 CLK1  
Two-hybrid Homo sapiens
8 SMURF1 57154
Affinity Capture-Luminescence Homo sapiens
9 Atg16l1  
Affinity Capture-MS Mus musculus
10 LUC7L 55692
Two-hybrid Homo sapiens
11 Fancd2  
Affinity Capture-MS Mus musculus
12 Tra2b 20462
Two-hybrid Mus musculus
View the network image/svg+xml



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