Gene description for Tra2b
Gene name transformer 2 beta homolog (Drosophila)
Gene symbol Tra2b
Other names/aliases 5730405G21Rik
D16Ertd266e
SIG-41
Sfrs10
Silg41
TRA2beta
Species Mus musculus
 Database cross references - Tra2b
ExoCarta ExoCarta_20462
Vesiclepedia VP_20462
Entrez Gene 20462
UniProt P62996  
 Tra2b identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Tra2b
Molecular Function
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 IEA
    RNA binding GO:0003723 ISO
    mRNA binding GO:0003729 ISO
    mRNA binding GO:0003729 ISS
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    pre-mRNA binding GO:0036002 IEA
    pre-mRNA binding GO:0036002 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 ISO
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 ISS
    mRNA splicing, via spliceosome GO:0000398 IMP
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
    cerebral cortex regionalization GO:0021796 IMP
    regulation of RNA splicing GO:0043484 IMP
    regulation of RNA splicing GO:0043484 ISO
    positive regulation of mRNA splicing, via spliceosome GO:0048026 IBA
    positive regulation of mRNA splicing, via spliceosome GO:0048026 IMP
    positive regulation of mRNA splicing, via spliceosome GO:0048026 ISO
    positive regulation of mRNA splicing, via spliceosome GO:0048026 ISS
    cellular response to glucose stimulus GO:0071333 IEA
    cellular response to glucose stimulus GO:0071333 ISO
    embryonic brain development GO:1990403 IMP
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nuclear inner membrane GO:0005637 IDA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    spliceosomal complex GO:0005681 IBA
    spliceosomal complex GO:0005681 IEA
    spliceosomal complex GO:0005681 ISO
    protein-containing complex GO:0032991 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Tra2b in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Tra2b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SRSF4 6429
Two-hybrid Homo sapiens
2 NXF1 10482
Two-hybrid Homo sapiens
3 Fmr1  
Affinity Capture-MS Mus musculus
4 Srsf9  
Two-hybrid Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Srsf3 20383
Two-hybrid Mus musculus
7 Atxn1  
Affinity Capture-MS Mus musculus
8 Tia1  
Affinity Capture-MS Mus musculus
9 Srsf5 20384
Two-hybrid Mus musculus
10 Unk  
Affinity Capture-RNA Mus musculus
11 CLK3  
Two-hybrid Homo sapiens
12 Fancd2  
Affinity Capture-MS Mus musculus
13 Nono 53610
Two-hybrid Mus musculus
14 RBMX 27316
Two-hybrid Homo sapiens
View the network image/svg+xml



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