Experiment description of studies that identified Nono in sEVs

1 Experiment ID 907 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample Colon cancer cells Sample name C26 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 15 MISEV standards ✔ EM Biophysical techniques ✔ CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule mrna Identification method Microarray PubMed ID 17486113     Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO" Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array

3 Experiment ID 188 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|UCHL1|HSP90 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19351151     Organism Mus musculus Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells. Authors "Lee HS, Jeong J, Lee KJ." Journal name J Proteome Res Publication year 2009 Sample Pancreatic cells Sample name Pancreatic beta cell (NIT-1) Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 908 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample T-cell lymphoma cells Sample name EL4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Nono

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Nufip2   Two-hybrid Mus musculus 2 Hnrnph2 56258 Two-hybrid Mus musculus 3 KIF24   Two-hybrid Homo sapiens 4 SFPQ 6421 Two-hybrid Homo sapiens Co-purification Homo sapiens 5 Mapre1 13589 Two-hybrid Mus musculus 6 Pou5f1   Affinity Capture-MS Mus musculus 7 GSK3B 2932 Two-hybrid Homo sapiens 8 LARP4B 23185 Two-hybrid Homo sapiens 9 BYSL 705 Two-hybrid Homo sapiens 10 DMRTB1   Two-hybrid Homo sapiens 11 Cstf2t   Two-hybrid Mus musculus 12 CEP55 55165 Two-hybrid Homo sapiens 13 CD2BP2 10421 Two-hybrid Homo sapiens 14 DIS3L   Two-hybrid Homo sapiens 15 Atxn1   Proximity Label-MS Mus musculus 16 SNRPA 6626 Two-hybrid Homo sapiens 17 Rbm14   Co-fractionation Mus musculus Two-hybrid Mus musculus 18 Smarca2   Affinity Capture-MS Mus musculus 19 CPNE4 131034 Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens 20 Tfe3   Affinity Capture-MS Mus musculus 21 CTTN 2017 Two-hybrid Homo sapiens 22 Smarca4   Affinity Capture-MS Mus musculus 23 Rpl18a 76808 Two-hybrid Mus musculus 24 CTBP1 1487 Two-hybrid Homo sapiens 25 Ywhae 22627 Affinity Capture-MS Mus musculus 26 MYO1C 4641 Two-hybrid Homo sapiens 27 RBM5   Two-hybrid Homo sapiens 28 Mettl21e   Affinity Capture-MS Mus musculus 29 GSK3A 2931 Two-hybrid Homo sapiens 30 Fzr1   Affinity Capture-MS Mus musculus 31 NOL7   Two-hybrid Homo sapiens 32 Per1   Affinity Capture-MS Mus musculus 33 USO1 8615 Two-hybrid Homo sapiens 34 Nanog   Affinity Capture-MS Mus musculus 35 Tra2b 20462 Two-hybrid Mus musculus 36 CPSF7 79869 Two-hybrid Homo sapiens 37 RPL23A 6147 Two-hybrid Homo sapiens 38 LYAR 55646 Two-hybrid Homo sapiens 39 CPNE1 8904 Reconstituted Complex Homo sapiens 40 Eed   Affinity Capture-MS Mus musculus 41 Ewsr1 14030 Two-hybrid Mus musculus 42 TFIP11   Two-hybrid Homo sapiens 43 Nudt21 68219 Two-hybrid Mus musculus 44 Ube2i   Two-hybrid Mus musculus 45 Pspc1   Two-hybrid Mus musculus 46 Atg16l1   Affinity Capture-MS Mus musculus 47 Cpsf6   Two-hybrid Mus musculus 48 BAG4   Two-hybrid Homo sapiens 49 Fancd2   Affinity Capture-MS Mus musculus 50 Tcf3   Affinity Capture-MS Mus musculus 51 Per2   Affinity Capture-MS Mus musculus View the network

Pathways in which Nono is involved

No pathways found