Experiment description of studies that identified Ssb in sEVs

1 Experiment ID 907 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample Colon cancer cells Sample name C26 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 15 MISEV standards ✔ EM Biophysical techniques ✔ CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17486113     Organism Mus musculus Homo sapiens Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO" Journal name NCB Publication year 2007 Sample Mast cells Sample name MC9 Bone marrow-derived mast cells HMC-1 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.11-1.21 g/mL Molecules identified in the study Protein mRNA miRNA Methods used in the study Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array

3 Experiment ID 908 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a Enriched markers ✔ HSPA5 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 37309723     Organism Mus musculus Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S." Journal name Proteomics Publication year 2023 Sample T-cell lymphoma cells Sample name EL4 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for Ssb

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Elp2   Co-fractionation Mus musculus 2 Trmt11   Co-fractionation Mus musculus 3 Polr3h   Co-fractionation Mus musculus 4 Snrpd1 20641 Co-fractionation Mus musculus 5 Impdh1   Co-fractionation Mus musculus 6 Trove2 20822 Co-fractionation Mus musculus 7 Kctd13   Affinity Capture-MS Mus musculus 8 Rsl1d1 66409 Co-fractionation Mus musculus 9 Ywhae 22627 Co-fractionation Mus musculus 10 Emd   Co-fractionation Mus musculus 11 Pabpc1 18458 Co-fractionation Mus musculus 12 Ddx47   Co-fractionation Mus musculus 13 Polr1c   Co-fractionation Mus musculus 14 Mettl10   Co-fractionation Mus musculus 15 3110082I17Rik   Co-fractionation Mus musculus 16 Sqstm1   Affinity Capture-MS Mus musculus 17 Vbp1   Co-fractionation Mus musculus 18 Eed   Affinity Capture-MS Mus musculus 19 Uba2 50995 Co-fractionation Mus musculus 20 Atg12   Positive Genetic Mus musculus 21 Fancd2   Affinity Capture-MS Mus musculus 22 Bccip   Co-fractionation Mus musculus 23 Pwp1   Co-fractionation Mus musculus View the network

Pathways in which Ssb is involved

No pathways found