Gene description for Eif4g1
Gene name eukaryotic translation initiation factor 4, gamma 1
Gene symbol Eif4g1
Other names/aliases E030015G23Rik
eIF-4-gamma 1
eIF-4G 1
eIF-4G1
eIF4GI
Species Mus musculus
 Database cross references - Eif4g1
ExoCarta ExoCarta_208643
Vesiclepedia VP_208643
Entrez Gene 208643
UniProt Q6NZJ6  
 Eif4g1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif4g1
Molecular Function
    mRNA binding GO:0003729 IBA
    mRNA binding GO:0003729 IEA
    mRNA binding GO:0003729 ISO
    translation initiation factor activity GO:0003743 IBA
    translation initiation factor activity GO:0003743 IEA
    translation initiation factor activity GO:0003743 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    translation factor activity, RNA binding GO:0008135 ISO
    eukaryotic initiation factor 4E binding GO:0008190 IEA
    eukaryotic initiation factor 4E binding GO:0008190 ISO
Biological Process
    behavioral fear response GO:0001662 IGI
    translation GO:0006412 ISO
    translational initiation GO:0006413 IBA
    translational initiation GO:0006413 ISO
    translational initiation GO:0006413 NAS
    regulation of translational initiation GO:0006446 IEA
    regulation of translational initiation GO:0006446 ISO
    macromolecule biosynthetic process GO:0009059 ISO
    negative regulation of autophagy GO:0010507 IEA
    negative regulation of autophagy GO:0010507 ISO
    negative regulation of peptidyl-threonine phosphorylation GO:0010801 ISO
    neuron differentiation GO:0030182 IMP
    positive regulation of cell growth GO:0030307 IEA
    positive regulation of cell growth GO:0030307 ISO
    lung development GO:0030324 IEA
    cellular response to nutrient levels GO:0031669 IEA
    cellular response to nutrient levels GO:0031669 ISO
    positive regulation of peptidyl-serine phosphorylation GO:0033138 ISO
    miRNA-mediated gene silencing by inhibition of translation GO:0035278 IEA
    miRNA-mediated gene silencing by inhibition of translation GO:0035278 ISO
    positive regulation of translation in response to endoplasmic reticulum stress GO:0036493 ISO
    positive regulation of translation in response to endoplasmic reticulum stress GO:0036493 ISS
    response to ethanol GO:0045471 IEA
    positive regulation of neuron differentiation GO:0045666 IMP
    positive regulation of protein metabolic process GO:0051247 ISO
    energy homeostasis GO:0097009 IEA
    energy homeostasis GO:0097009 ISO
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 IEA
    positive regulation of G1/S transition of mitotic cell cycle GO:1900087 ISO
    positive regulation of protein localization to cell periphery GO:1904377 IEA
    positive regulation of protein localization to cell periphery GO:1904377 ISO
    positive regulation of eukaryotic translation initiation factor 4F complex assembly GO:1905537 IEA
    positive regulation of eukaryotic translation initiation factor 4F complex assembly GO:1905537 ISO
    regulation of presynapse assembly GO:1905606 IEA
    regulation of presynapse assembly GO:1905606 ISO
    positive regulation of mRNA cap binding GO:1905612 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    ribosome GO:0005840 IEA
    ribosome GO:0005840 ISO
    cytoplasmic stress granule GO:0010494 ISO
    cytoplasmic stress granule GO:0010494 ISS
    eukaryotic translation initiation factor 4F complex GO:0016281 IBA
    eukaryotic translation initiation factor 4F complex GO:0016281 ISO
    eukaryotic translation initiation factor 4F complex GO:0016281 NAS
    postsynapse GO:0098794 IEA
    postsynapse GO:0098794 ISO
 Experiment description of studies that identified Eif4g1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif4g1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fzr1  
Affinity Capture-MS Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Hspa8 15481
Co-fractionation Mus musculus
5 Fmr1  
Affinity Capture-MS Mus musculus
6 Foxp3  
Affinity Capture-MS Mus musculus
7 Eed  
Affinity Capture-MS Mus musculus
8 MKRN1 23608
Affinity Capture-MS Homo sapiens
9 Iqgap1 29875
Co-fractionation Mus musculus
10 Eif4e  
Affinity Capture-Western Mus musculus
11 Atxn1  
Proximity Label-MS Mus musculus
12 Ddx3x 13205
Co-fractionation Mus musculus
13 Eef1e1  
Co-fractionation Mus musculus
14 Eif5 217869
Co-fractionation Mus musculus
15 Unk  
Affinity Capture-RNA Mus musculus
16 Larp1  
Co-fractionation Mus musculus
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