Gene description for Eif5
Gene name eukaryotic translation initiation factor 5
Gene symbol Eif5
Other names/aliases 2810011H21Rik
D12Ertd549e
Species Mus musculus
 Database cross references - Eif5
ExoCarta ExoCarta_217869
Vesiclepedia VP_217869
Entrez Gene 217869
UniProt P59325  
 Eif5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif5
Molecular Function
    translation initiation factor activity GO:0003743 IBA
    translation initiation factor activity GO:0003743 IEA
    translation initiation factor activity GO:0003743 ISO
    GDP-dissociation inhibitor activity GO:0005092 IBA
    GTPase activator activity GO:0005096 IEA
    GTP binding GO:0005525 IEA
    eukaryotic initiation factor eIF2 binding GO:0071074 IBA
    eukaryotic initiation factor eIF2 binding GO:0071074 ISO
Biological Process
    formation of translation preinitiation complex GO:0001731 ISO
    formation of cytoplasmic translation initiation complex GO:0001732 IBA
    translational initiation GO:0006413 IEA
    regulation of translational initiation GO:0006446 IEA
    regulation of translational initiation GO:0006446 ISO
    ribosome assembly GO:0042255 IEA
    ribosome assembly GO:0042255 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
 Experiment description of studies that identified Eif5 in sEVs
1
Experiment ID 907
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PA2G4 5036
Two-hybrid Homo sapiens
2 Eif4g1 208643
Co-fractionation Mus musculus
3 Scly  
Co-fractionation Mus musculus
4 Isoc1 66307
Co-fractionation Mus musculus
5 Tbcb 66411
Co-fractionation Mus musculus
6 Capns1  
Co-fractionation Mus musculus
7 Fitm2  
Positive Genetic Mus musculus
8 Snrpd1 20641
Co-fractionation Mus musculus
9 Atg16l1  
Affinity Capture-MS Mus musculus
10 Eif1 20918
Co-fractionation Mus musculus
11 Ubfd1  
Co-fractionation Mus musculus
12 Stk24 223255
Co-fractionation Mus musculus
13 Ywhae 22627
Co-fractionation Mus musculus
View the network image/svg+xml



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