Gene description for Stk24
Gene name serine/threonine kinase 24
Gene symbol Stk24
Other names/aliases 1810013H02Rik
C76483
MST-3
Mst3
STE20
Species Mus musculus
 Database cross references - Stk24
ExoCarta ExoCarta_223255
Vesiclepedia VP_223255
Entrez Gene 223255
UniProt Q99KH8  
 Stk24 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Stk24
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 ISO
    protein serine/threonine kinase activity GO:0004674 ISS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 ISO
    protein phosphorylation GO:0006468 ISS
    intrinsic apoptotic signaling pathway in response to oxidative stress GO:0008631 ISO
    intrinsic apoptotic signaling pathway in response to oxidative stress GO:0008631 ISS
    cellular response to starvation GO:0009267 ISO
    cellular response to starvation GO:0009267 ISS
    negative regulation of cell migration GO:0030336 IBA
    negative regulation of cell migration GO:0030336 ISO
    negative regulation of cell migration GO:0030336 ISS
    cellular response to oxidative stress GO:0034599 IEA
    cellular response to oxidative stress GO:0034599 ISO
    intracellular signal transduction GO:0035556 IBA
    protein autophosphorylation GO:0046777 ISO
    protein autophosphorylation GO:0046777 ISS
    regulation of axon regeneration GO:0048679 ISO
    positive regulation of axon regeneration GO:0048680 IEA
    positive regulation of axon regeneration GO:0048680 ISO
    execution phase of apoptosis GO:0097194 IEA
    execution phase of apoptosis GO:0097194 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi membrane GO:0000139 ISO
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    Golgi apparatus GO:0005794 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    FAR/SIN/STRIPAK complex GO:0090443 ISO
    FAR/SIN/STRIPAK complex GO:0090443 ISS
 Experiment description of studies that identified Stk24 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Stk24
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Msn 17698
Co-fractionation Mus musculus
2 Sharpin  
Affinity Capture-MS Mus musculus
3 Cttnbp2  
Co-fractionation Mus musculus
4 Pdia4 12304
Co-fractionation Mus musculus
5 Ppp2ca 19052
Co-fractionation Mus musculus
6 Isoc1 66307
Co-fractionation Mus musculus
7 Tbcb 66411
Co-fractionation Mus musculus
8 Scpep1  
Co-fractionation Mus musculus
9 Strn3  
Co-fractionation Mus musculus
10 Cct8 12469
Co-fractionation Mus musculus
11 Prcp  
Co-fractionation Mus musculus
12 Pdcd10 56426
Co-fractionation Mus musculus
13 Eif5 217869
Co-fractionation Mus musculus
14 Fancd2  
Affinity Capture-MS Mus musculus
15 Cct7 12468
Co-fractionation Mus musculus
16 Asns 27053
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Stk24 is involved
PathwayEvidenceSource
Apoptosis IEA Reactome
Apoptotic cleavage of cellular proteins IEA Reactome
Apoptotic execution phase IEA Reactome
Programmed Cell Death IEA Reactome





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