Gene description for Cct8
Gene name chaperonin containing Tcp1, subunit 8 (theta)
Gene symbol Cct8
Other names/aliases AI132397
Cctq
Tcpq
Species Mus musculus
 Database cross references - Cct8
ExoCarta ExoCarta_12469
Vesiclepedia VP_12469
Entrez Gene 12469
UniProt P42932  
 Cct8 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Neural stem cells 25242146    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cct8
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    protein folding chaperone GO:0044183 ISO
    unfolded protein binding GO:0051082 IBA
    unfolded protein binding GO:0051082 IEA
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    protein folding GO:0006457 IBA
    protein folding GO:0006457 ISO
    binding of sperm to zona pellucida GO:0007339 IDA
    positive regulation of telomere maintenance via telomerase GO:0032212 IEA
    positive regulation of telomere maintenance via telomerase GO:0032212 ISO
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    chaperone mediated protein folding independent of cofactor GO:0051086 IEA
    chaperone-mediated protein folding GO:0061077 ISO
    positive regulation of establishment of protein localization to telomere GO:1904851 ISO
Subcellular Localization
    zona pellucida receptor complex GO:0002199 IDA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 ISO
    cytosol GO:0005829 ISO
    chaperonin-containing T-complex GO:0005832 IBA
    chaperonin-containing T-complex GO:0005832 IDA
    chaperonin-containing T-complex GO:0005832 ISO
    microtubule GO:0005874 IEA
    microtubule GO:0005874 ISO
    cilium GO:0005929 IEA
    cell body GO:0044297 IDA
    intermediate filament cytoskeleton GO:0045111 IEA
    intermediate filament cytoskeleton GO:0045111 ISO
 Experiment description of studies that identified Cct8 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
7
Experiment ID 264
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cct8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TMCO1 54499
Affinity Capture-MS Homo sapiens
2 MLST8 64223
Affinity Capture-MS Homo sapiens
3 Elf5  
Affinity Capture-MS Mus musculus
4 DTL  
Affinity Capture-MS Homo sapiens
5 TUBB 203068
Affinity Capture-MS Homo sapiens
6 Strn3  
Co-fractionation Mus musculus
7 CCT3 7203
Affinity Capture-MS Homo sapiens
8 PDCL3 79031
Affinity Capture-MS Homo sapiens
9 CSTF1 1477
Affinity Capture-MS Homo sapiens
10 NDUFA13 51079
Affinity Capture-MS Homo sapiens
11 Ppp2r2a 71978
Co-fractionation Mus musculus
12 EIF3I 8668
Affinity Capture-MS Homo sapiens
13 TNPO1 3842
Affinity Capture-MS Homo sapiens
14 DHX15 1665
Affinity Capture-MS Homo sapiens
15 PDCL 5082
Affinity Capture-MS Homo sapiens
16 CCT6A 908
Affinity Capture-MS Homo sapiens
17 Tsc1  
Affinity Capture-MS Mus musculus
18 Atxn1  
Proximity Label-MS Mus musculus
19 TCP1 6950
Affinity Capture-MS Homo sapiens
20 Kctd13  
Affinity Capture-MS Mus musculus
21 SLC2A1 6513
Affinity Capture-MS Homo sapiens
22 Tfe3  
Affinity Capture-MS Mus musculus
23 CCT4 10575
Affinity Capture-MS Homo sapiens
24 Set 56086
Affinity Capture-MS Mus musculus
25 CCT2 10576
Affinity Capture-MS Homo sapiens
26 HGH1 51236
Affinity Capture-MS Homo sapiens
27 CCT7 10574
Affinity Capture-MS Homo sapiens
28 TXNDC9 10190
Affinity Capture-MS Homo sapiens
29 CCT5 22948
Affinity Capture-MS Homo sapiens
30 Tmem173  
Proximity Label-MS Mus musculus
31 Fzr1  
Affinity Capture-MS Mus musculus
32 ACTR2 10097
Affinity Capture-MS Homo sapiens
33 ATP13A2  
Affinity Capture-MS Homo sapiens
34 PFDN2 5202
Affinity Capture-MS Homo sapiens
35 KIAA2013 90231
Affinity Capture-MS Homo sapiens
36 Atg5  
Affinity Capture-RNA Mus musculus
37 ESCO1  
Affinity Capture-MS Homo sapiens
38 PDCD5 9141
Affinity Capture-MS Homo sapiens
39 ACTL8  
Affinity Capture-MS Homo sapiens
40 Unk  
Affinity Capture-RNA Mus musculus
41 HACE1 57531
Affinity Capture-MS Homo sapiens
42 NCLN 56926
Affinity Capture-MS Homo sapiens
43 PPP6C 5537
Affinity Capture-MS Homo sapiens
44 RFWD3  
Affinity Capture-MS Homo sapiens
45 ACTB 60
Reconstituted Complex Homo sapiens
46 WDR92 116143
Affinity Capture-MS Homo sapiens
47 Eed  
Affinity Capture-MS Mus musculus
48 Ahi1  
Affinity Capture-MS Mus musculus
49 Atg16l1  
Affinity Capture-MS Mus musculus
50 Ppp2r2d  
Co-fractionation Mus musculus
51 Stk24 223255
Co-fractionation Mus musculus
52 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here