Gene description for Asns
Gene name asparagine synthetase
Gene symbol Asns
Other names/aliases -
Species Mus musculus
 Database cross references - Asns
ExoCarta ExoCarta_27053
Vesiclepedia VP_27053
Entrez Gene 27053
UniProt Q61024  
 Asns identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Asns
Molecular Function
    asparagine synthase (glutamine-hydrolyzing) activity GO:0004066 IBA
    asparagine synthase (glutamine-hydrolyzing) activity GO:0004066 ISO
    ATP binding GO:0005524 IEA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    liver development GO:0001889 IEA
    asparagine biosynthetic process GO:0006529 IBA
    asparagine biosynthetic process GO:0006529 ISO
    asparagine biosynthetic process GO:0006529 ISO
    response to light stimulus GO:0009416 IEA
    response to mechanical stimulus GO:0009612 IEA
    response to toxic substance GO:0009636 IEA
    response to follicle-stimulating hormone GO:0032354 IEA
    cellular response to hormone stimulus GO:0032870 IEA
    cellular response to glucose starvation GO:0042149 IEA
    cellular response to glucose starvation GO:0042149 ISO
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    response to amino acid GO:0043200 IEA
    positive regulation of mitotic cell cycle GO:0045931 IEA
    positive regulation of mitotic cell cycle GO:0045931 ISO
    L-asparagine biosynthetic process GO:0070981 IEA
    response to aldosterone GO:1904044 IEA
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
 Experiment description of studies that identified Asns in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Asns
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ogfod1  
Co-fractionation Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Ptma  
Co-fractionation Mus musculus
4 Eed  
Affinity Capture-MS Mus musculus
5 Tfe3  
Affinity Capture-MS Mus musculus
6 Twf2  
Co-fractionation Mus musculus
7 Stk24 223255
Co-fractionation Mus musculus
8 Fancd2  
Affinity Capture-MS Mus musculus
9 Ywhae 22627
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Asns is involved
PathwayEvidenceSource
Aspartate and asparagine metabolism IEA Reactome
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome





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