Gene description for Dpysl3
Gene name dihydropyrimidinase-like 3
Gene symbol Dpysl3
Other names/aliases CRMP-4
DRP-3
TUC4
ULIP-1
Ulip
Ulip1
Species Mus musculus
 Database cross references - Dpysl3
ExoCarta ExoCarta_22240
Vesiclepedia VP_22240
Entrez Gene 22240
UniProt Q62188  
 Dpysl3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Oligodendrocytes 21136642    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dpysl3
Molecular Function
    dihydropyrimidinase activity GO:0004157 IBA
    protein binding GO:0005515 IPI
    hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds GO:0016810 IEA
    hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides GO:0016812 IBA
    SH3 domain binding GO:0017124 ISO
    SH3 domain binding GO:0017124 ISS
    filamin binding GO:0031005 ISO
    chondroitin sulfate binding GO:0035374 ISO
    chondroitin sulfate binding GO:0035374 ISS
    identical protein binding GO:0042802 ISO
    identical protein binding GO:0042802 ISS
    phosphoprotein binding GO:0051219 IPI
Biological Process
    pyrimidine nucleobase catabolic process GO:0006208 IBA
    nervous system development GO:0007399 IDA
    positive regulation of neuron projection development GO:0010976 ISO
    positive regulation of neuron projection development GO:0010976 ISS
    negative regulation of neuron projection development GO:0010977 ISO
    negative regulation of neuron projection development GO:0010977 ISS
    negative regulation of cell migration GO:0030336 ISO
    negative regulation of cell migration GO:0030336 ISS
    response to axon injury GO:0048678 ISS
    actin filament bundle assembly GO:0051017 ISO
    actin filament bundle assembly GO:0051017 ISS
    positive regulation of filopodium assembly GO:0051491 ISO
    positive regulation of filopodium assembly GO:0051491 ISS
    actin crosslink formation GO:0051764 IBA
    actin crosslink formation GO:0051764 ISO
    actin crosslink formation GO:0051764 ISS
    cellular response to cytokine stimulus GO:0071345 ISS
Subcellular Localization
    extracellular space GO:0005615 ISO
    extracellular space GO:0005615 ISS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    lamellipodium GO:0030027 ISO
    lamellipodium GO:0030027 ISS
    growth cone GO:0030426 ISO
    growth cone GO:0030426 ISS
    filamentous actin GO:0031941 ISO
    filamentous actin GO:0031941 ISS
    cell body GO:0044297 ISO
    cell body GO:0044297 ISS
    synapse GO:0045202 ISO
    exocytic vesicle GO:0070382 ISO
    exocytic vesicle GO:0070382 ISS
 Experiment description of studies that identified Dpysl3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dpysl3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 St13 70356
Co-fractionation Mus musculus
2 Xrcc6  
Co-fractionation Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Mapt  
Affinity Capture-MS Mus musculus
5 Tia1  
Affinity Capture-MS Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
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