Gene description for RPH3A
Gene name rabphilin 3A
Gene symbol RPH3A
Other names/aliases -
Species Homo sapiens
 Database cross references - RPH3A
ExoCarta ExoCarta_22895
Vesiclepedia VP_22895
Entrez Gene 22895
HGNC 17056
MIM 612159
UniProt Q9Y2J0  
 RPH3A identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for RPH3A
Molecular Function
    calcium ion binding GO:0005509 ISS
    protein binding GO:0005515 IPI
    calcium-dependent phospholipid binding GO:0005544 ISS
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 ISS
    zinc ion binding GO:0008270 ISS
    selenium binding GO:0008430 ISS
    small GTPase binding GO:0031267 IEA
    phosphate ion binding GO:0042301 ISS
    protein-containing complex binding GO:0044877 ISS
    inositol 1,4,5 trisphosphate binding GO:0070679 ISS
    phosphatidylinositol phosphate binding GO:1901981 TAS
Biological Process
    intracellular protein transport GO:0006886 IEA
    synaptic vesicle priming GO:0016082 IEA
    positive regulation of calcium ion-dependent exocytosis GO:0045956 IBA
    spontaneous neurotransmitter secretion GO:0061669 IEA
    calcium-dependent activation of synaptic vesicle fusion GO:0099502 IBA
Subcellular Localization
    Golgi apparatus GO:0005794 ISS
    cytosol GO:0005829 ISS
    synaptic vesicle GO:0008021 TAS
    extrinsic component of membrane GO:0019898 ISS
    secretory granule GO:0030141 ISS
    synaptic vesicle membrane GO:0030672 ISS
    protein-containing complex GO:0032991 ISS
    neuron projection GO:0043005 IBA
    neuron projection GO:0043005 ISS
    dendritic spine GO:0043197 IEA
    synapse GO:0045202 IBA
    synapse GO:0045202 ISS
    postsynaptic membrane GO:0045211 IBA
    extrinsic component of synaptic vesicle membrane GO:0098850 IBA
 Experiment description of studies that identified RPH3A in sEVs
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for RPH3A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Reconstituted Complex Homo sapiens
2 RAB8B 51762
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 DOC2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 APPL1 26060
Affinity Capture-MS Homo sapiens
5 PRNP 5621
Affinity Capture-MS Homo sapiens
6 CASK 8573
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 ALOX15B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 UBE3A 7337
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 SNAP25 6616
Affinity Capture-Western Homo sapiens
10 CAND1 55832
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
11 PARK2  
Affinity Capture-MS Homo sapiens
12 SYDE1 85360
Affinity Capture-MS Homo sapiens
13 RAB8A 4218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 GCH1  
Affinity Capture-Western Homo sapiens
15 ADD2 119
Reconstituted Complex Homo sapiens
16 NTRK1 4914
Affinity Capture-MS Homo sapiens
17 MPP5 64398
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 TP63  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 RAB3B 5865
Reconstituted Complex Homo sapiens
20 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
21 RAB3A 5864
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
22 RAB27A 5873
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RPH3A is involved
No pathways found





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