Gene description for TBC1D9B
Gene name TBC1 domain family, member 9B (with GRAM domain)
Gene symbol TBC1D9B
Other names/aliases -
Species Homo sapiens
 Database cross references - TBC1D9B
ExoCarta ExoCarta_23061
Vesiclepedia VP_23061
Entrez Gene 23061
HGNC 29097
UniProt Q66K14  
 TBC1D9B identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for TBC1D9B
Molecular Function
    GTPase activator activity GO:0005096 IBA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
Biological Process
    regulation of cilium assembly GO:1902017 IMP
Subcellular Localization
    membrane GO:0016020 IEA
 Experiment description of studies that identified TBC1D9B in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TBC1D9B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
2 GABARAPL1 23710
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 F2RL1  
Affinity Capture-MS Homo sapiens
4 EP400NL  
Affinity Capture-MS Homo sapiens
5 ARL8A 127829
Affinity Capture-MS Homo sapiens
6 Batf3  
Affinity Capture-MS Mus musculus
7 ALDH3A2 224
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 VAPB 9217
Affinity Capture-MS Homo sapiens
9 GPR17 2840
Affinity Capture-MS Homo sapiens
10 SIGLECL1  
Affinity Capture-MS Homo sapiens
11 BTNL9  
Affinity Capture-MS Homo sapiens
12 FPR1  
Affinity Capture-MS Homo sapiens
13 Dync1li1 235661
Affinity Capture-MS Mus musculus
14 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 LRRC55  
Affinity Capture-MS Homo sapiens
16 CLK2 1196
Affinity Capture-MS Homo sapiens
17 ARL4D  
Affinity Capture-MS Homo sapiens
18 TYROBP  
Affinity Capture-MS Homo sapiens
19 FAM43A  
Affinity Capture-MS Homo sapiens
20 EVA1C  
Affinity Capture-MS Homo sapiens
21 GABARAPL2 11345
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 CUL3 8452
Affinity Capture-MS Homo sapiens
23 LANCL1 10314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 EFNB1 1947
Affinity Capture-MS Homo sapiens
25 C19orf38  
Affinity Capture-MS Homo sapiens
26 LAMP2 3920
Proximity Label-MS Homo sapiens
27 BPNT1 10380
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CTAG2  
Affinity Capture-MS Homo sapiens
29 AQP3  
Affinity Capture-MS Homo sapiens
30 TMEM55B 90809
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 TMEM108  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 ABHD12 26090
Affinity Capture-MS Homo sapiens
33 TBC1D9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 ILVBL 10994
Affinity Capture-MS Homo sapiens
35 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
36 MAP1LC3B 81631
Two-hybrid Homo sapiens
37 COMTD1 118881
Affinity Capture-MS Homo sapiens
38 MAP1LC3A 84557
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
39 DHFR 1719
Affinity Capture-MS Homo sapiens
40 MYO1D 4642
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 AMPD2 271
Co-fractionation Homo sapiens
42 RPA3 6119
Proximity Label-MS Homo sapiens
43 CRLF1 9244
Affinity Capture-MS Homo sapiens
44 VCP 7415
Affinity Capture-MS Homo sapiens
45 GPR45  
Affinity Capture-MS Homo sapiens
46 RAB9A 9367
Proximity Label-MS Homo sapiens
47 METTL21B  
Affinity Capture-MS Homo sapiens
48 FZD10  
Affinity Capture-MS Homo sapiens
49 ARRDC3 57561
Affinity Capture-MS Homo sapiens
50 CLEC2B  
Affinity Capture-MS Homo sapiens
51 KIR2DL4  
Affinity Capture-MS Homo sapiens
52 L3MBTL2  
Two-hybrid Homo sapiens
53 KLRC1  
Affinity Capture-MS Homo sapiens
54 FAXC  
Affinity Capture-MS Homo sapiens
55 SEMA4C 54910
Affinity Capture-MS Homo sapiens
56 SPINT2 10653
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 SEC62 7095
Affinity Capture-MS Homo sapiens
58 RABL2A 11159
Co-fractionation Homo sapiens
59 CEBPD  
Co-fractionation Homo sapiens
60 TACSTD2 4070
Affinity Capture-MS Homo sapiens
61 S1PR4  
Affinity Capture-MS Homo sapiens
62 DKKL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 ADORA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TBC1D9B is involved
No pathways found





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