Gene description for Cnot3
Gene name CCR4-NOT transcription complex, subunit 3
Gene symbol Cnot3
Other names/aliases A930039N10Rik
Species Mus musculus
 Database cross references - Cnot3
ExoCarta ExoCarta_232791
Vesiclepedia VP_232791
Entrez Gene 232791
UniProt Q8K0V4  
 Cnot3 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Cnot3
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    nuclear-transcribed mRNA poly(A) tail shortening GO:0000289 IBA
    trophectodermal cell differentiation GO:0001829 IMP
    regulation of DNA-templated transcription GO:0006355 IEA
    regulation of translation GO:0006417 IEA
    regulatory ncRNA-mediated gene silencing GO:0031047 IEA
    negative regulation of intracellular estrogen receptor signaling pathway GO:0033147 ISS
    positive regulation of cold-induced thermogenesis GO:0120162 IMP
    regulation of stem cell population maintenance GO:2000036 IBA
    regulation of stem cell population maintenance GO:2000036 IMP
    regulation of stem cell population maintenance GO:2000036 ISO
Subcellular Localization
    P-body GO:0000932 IBA
    P-body GO:0000932 IDA
    nucleus GO:0005634 IEA
    cytosol GO:0005829 TAS
    CCR4-NOT complex GO:0030014 ISO
    CCR4-NOT complex GO:0030014 ISS
    CCR4-NOT core complex GO:0030015 IBA
    CCR4-NOT core complex GO:0030015 IEA
 Experiment description of studies that identified Cnot3 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Cnot3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CNOT11 55571
Affinity Capture-MS Homo sapiens
2 TNKS1BP1 85456
Affinity Capture-MS Homo sapiens
3 CNOT7 29883
Affinity Capture-MS Homo sapiens
4 Atxn1  
Affinity Capture-MS Mus musculus
5 RQCD1 9125
Affinity Capture-MS Homo sapiens
6 Foxp3  
Affinity Capture-MS Mus musculus
7 Crebbp  
Two-hybrid Mus musculus
Reconstituted Complex Mus musculus
8 CNOT1 23019
Affinity Capture-MS Homo sapiens
9 RNF219  
Affinity Capture-MS Homo sapiens
10 TOB2  
Affinity Capture-MS Homo sapiens
11 CNOT10 25904
Affinity Capture-MS Homo sapiens
12 RAVER1 125950
Affinity Capture-MS Homo sapiens
13 CNOT8  
Affinity Capture-MS Homo sapiens
14 FHL2 2274
Affinity Capture-MS Homo sapiens
15 CNOT6  
Affinity Capture-MS Homo sapiens
16 CNOT3 4849
Affinity Capture-MS Homo sapiens
17 CNOT6L 246175
Affinity Capture-MS Homo sapiens
18 BTG3  
Affinity Capture-MS Homo sapiens
19 CNOT2  
Affinity Capture-MS Homo sapiens
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