Gene description for Spon1
Gene name spondin 1, (f-spondin) extracellular matrix protein
Gene symbol Spon1
Other names/aliases AI666765
AW455831
BC020531
D330035F22Rik
FSP
Species Mus musculus
 Database cross references - Spon1
ExoCarta ExoCarta_233744
Vesiclepedia VP_233744
Entrez Gene 233744
UniProt Q8VCC9  
 Spon1 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Spon1
Molecular Function
    extracellular matrix structural constituent GO:0005201 RCA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    LBD domain binding GO:0050693 IPI
Biological Process
    cell adhesion GO:0007155 IBA
    cell adhesion GO:0007155 IEA
    positive regulation of protein processing GO:0010954 IDA
    protein processing GO:0016485 IDA
    positive regulation of protein binding GO:0032092 IDA
    negative regulation of amyloid-beta formation GO:1902430 IDA
    positive regulation of amyloid precursor protein catabolic process GO:1902993 IDA
Subcellular Localization
    extracellular region GO:0005576 IEA
    extracellular matrix GO:0031012 IBA
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified Spon1 in sEVs
1
Experiment ID 15
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Spon1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Aplp1 11803
Co-fractionation Mus musculus
2 Kctd13  
Affinity Capture-MS Mus musculus
3 App 11820
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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