Gene description for ARHGAP8
Gene name Rho GTPase activating protein 8
Gene symbol ARHGAP8
Other names/aliases BPGAP1
PP610
Species Homo sapiens
 Database cross references - ARHGAP8
ExoCarta ExoCarta_23779
Vesiclepedia VP_23779
Entrez Gene 23779
HGNC 677
MIM 609405
UniProt P85298  
 ARHGAP8 identified in sEVs derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ARHGAP8
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 IDA
    GTPase activator activity GO:0005096 TAS
    protein binding GO:0005515 IPI
Biological Process
    small GTPase-mediated signal transduction GO:0007264 IBA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IDA
    negative regulation of endocytic recycling GO:2001136 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ARHGAP8 in sEVs
1
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARHGAP8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SH3GL1 6455
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 PRR5-ARHGAP8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CALML3 810
Affinity Capture-MS Homo sapiens
4 RBM43 375287
Affinity Capture-MS Homo sapiens
5 SH3GL3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 HTR3C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 ISOC1 51015
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 NOXO1  
Affinity Capture-MS Homo sapiens
9 ARHGAP8 23779
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SQSTM1 8878
Proximity Label-MS Homo sapiens
11 FEM1A  
Affinity Capture-MS Homo sapiens
12 CYGB  
Two-hybrid Homo sapiens
13 CALM1 801
Affinity Capture-MS Homo sapiens
14 SH3GL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 LRRC10  
Affinity Capture-MS Homo sapiens
16 ATG9A 79065
Proximity Label-MS Homo sapiens
17 DDX58 23586
Affinity Capture-RNA Homo sapiens
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