Gene description for KIF4A
Gene name kinesin family member 4A
Gene symbol KIF4A
Other names/aliases KIF4
KIF4G1
MRX100
Species Homo sapiens
 Database cross references - KIF4A
ExoCarta ExoCarta_24137
Vesiclepedia VP_24137
Entrez Gene 24137
HGNC 13339
MIM 300521
UniProt O95239  
 KIF4A identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Melanoma cells 25950383    
 Gene ontology annotations for KIF4A
Molecular Function
    DNA binding GO:0003677 IEA
    microtubule motor activity GO:0003777 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IEA
    metal ion binding GO:0046872 IEA
    iron-sulfur cluster binding GO:0051536 IEA
Biological Process
    mitotic cytokinesis GO:0000281 IMP
    organelle organization GO:0006996 TAS
    mitotic spindle organization GO:0007052 IBA
    anterograde axonal transport GO:0008089 TAS
    spindle elongation GO:0051231 IBA
    mitotic spindle midzone assembly GO:0051256 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    chromosome GO:0005694 IEA
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 TAS
    microtubule associated complex GO:0005875 IBA
    spindle microtubule GO:0005876 TAS
    membrane GO:0016020 HDA
    nuclear matrix GO:0016363 IEA
    midbody GO:0030496 IDA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified KIF4A in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for KIF4A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HMG20B  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
2 ZRANB1 54764
Affinity Capture-MS Homo sapiens
3 CCAR1 55749
Co-fractionation Homo sapiens
4 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
5 PICK1  
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 ZPR1 8882
Co-fractionation Homo sapiens
8 SIRT6  
Affinity Capture-MS Homo sapiens
9 PARP1 142
Proximity Label-MS Homo sapiens
10 BLOC1S6  
Affinity Capture-MS Homo sapiens
11 CENPA  
Proximity Label-MS Homo sapiens
12 TBC1D23 55773
Affinity Capture-MS Homo sapiens
13 KIF14 9928
Affinity Capture-MS Homo sapiens
14 SIN3A  
Co-fractionation Homo sapiens
15 SMC2 10592
Co-fractionation Homo sapiens
16 RNH1 6050
Co-fractionation Homo sapiens
17 SMARCA5 8467
Co-fractionation Homo sapiens
18 SIRT7  
Affinity Capture-MS Homo sapiens
19 BTF3 689
Affinity Capture-MS Homo sapiens
20 SMC4 10051
Co-fractionation Homo sapiens
21 NFATC1 4772
Affinity Capture-MS Homo sapiens
22 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
23 TUBB4B 10383
Affinity Capture-MS Homo sapiens
24 ACTC1 70
Proximity Label-MS Homo sapiens
25 CUL3 8452
Affinity Capture-MS Homo sapiens
26 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
27 NHLRC2 374354
Affinity Capture-MS Homo sapiens
28 EGFR 1956
Negative Genetic Homo sapiens
29 HDAC1 3065
Co-fractionation Homo sapiens
30 PARK2  
Affinity Capture-MS Homo sapiens
31 GBF1 8729
Co-fractionation Homo sapiens
32 KIF4B  
Affinity Capture-MS Homo sapiens
33 FAM9C  
Affinity Capture-MS Homo sapiens
34 APP 351
Reconstituted Complex Homo sapiens
35 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
36 NXF1 10482
Affinity Capture-RNA Homo sapiens
37 ACTR6  
Co-fractionation Homo sapiens
38 CBX3 11335
Proximity Label-MS Homo sapiens
39 BRCA2 675
Affinity Capture-Western Homo sapiens
40 MRFAP1L1  
Affinity Capture-MS Homo sapiens
41 NCAPH 23397
Affinity Capture-MS Homo sapiens
42 DNMT3B  
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
43 MOV10 4343
Affinity Capture-RNA Homo sapiens
44 RPA3 6119
Proximity Label-MS Homo sapiens
45 Kif4  
Affinity Capture-MS Mus musculus
46 TGFB1 7040
Affinity Capture-MS Homo sapiens
47 NTRK1 4914
Affinity Capture-MS Homo sapiens
48 PTRF 284119
Co-fractionation Homo sapiens
49 METTL21B  
Affinity Capture-MS Homo sapiens
50 DNPEP 23549
Affinity Capture-MS Homo sapiens
51 ANGPT4  
Affinity Capture-MS Homo sapiens
52 PRC1 9055
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
53 HIST1H1C 3006
Cross-Linking-MS (XL-MS) Homo sapiens
54 SNW1 22938
Affinity Capture-MS Homo sapiens
55 SPATA6L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 KRAS 3845
Synthetic Lethality Homo sapiens
57 ANAPC4 29945
Affinity Capture-MS Homo sapiens
58 AR 367
Proximity Label-MS Homo sapiens
59 HECTD1 25831
Affinity Capture-MS Homo sapiens
60 HAT1 8520
Co-fractionation Homo sapiens
61 HIST1H3A 8350
Proximity Label-MS Homo sapiens
62 NFATC2  
Affinity Capture-MS Homo sapiens
63 CEBPA  
Protein-peptide Homo sapiens
64 CDC5L 988
Affinity Capture-MS Homo sapiens
65 EED  
Affinity Capture-MS Homo sapiens
66 SLX4  
Affinity Capture-MS Homo sapiens
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