Gene description for RALGAPA1
Gene name Ral GTPase activating protein, alpha subunit 1 (catalytic)
Gene symbol RALGAPA1
Other names/aliases GARNL1
GRIPE
RalGAPalpha1
TULIP1
p240
Species Homo sapiens
 Database cross references - RALGAPA1
ExoCarta ExoCarta_253959
Vesiclepedia VP_253959
Entrez Gene 253959
HGNC 17770
MIM 608884
UniProt Q6GYQ0  
 RALGAPA1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for RALGAPA1
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 ISS
    protein heterodimerization activity GO:0046982 IPI
Biological Process
    regulation of small GTPase mediated signal transduction GO:0051056 IEA
    activation of GTPase activity GO:0090630 ISS
Subcellular Localization
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
 Experiment description of studies that identified RALGAPA1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RALGAPA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB35 11021
Proximity Label-MS Homo sapiens
2 AP2B1 163
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 TERF2  
Affinity Capture-MS Homo sapiens
4 SLMAP 7871
Proximity Label-MS Homo sapiens
5 OCLN 100506658
Proximity Label-MS Homo sapiens
6 LAMP3  
Proximity Label-MS Homo sapiens
7 DLG3 1741
Affinity Capture-MS Homo sapiens
8 LMAN1 3998
Proximity Label-MS Homo sapiens
9 RAB11A 8766
Proximity Label-MS Homo sapiens
10 LAMP1 3916
Proximity Label-MS Homo sapiens
11 GFOD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 KIF20A 10112
Affinity Capture-MS Homo sapiens
13 LAMP2 3920
Proximity Label-MS Homo sapiens
14 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 RAB4A 5867
Proximity Label-MS Homo sapiens
16 APEX1 328
Affinity Capture-RNA Homo sapiens
17 Rrp1b 72462
Affinity Capture-MS Mus musculus
18 DNAJC5 80331
Proximity Label-MS Homo sapiens
19 TERF1 7013
Affinity Capture-MS Homo sapiens
20 RAB2A 5862
Proximity Label-MS Homo sapiens
21 YWHAZ 7534
Affinity Capture-MS Homo sapiens
22 DIRAS3  
Proximity Label-MS Homo sapiens
23 DYRK1A 1859
Affinity Capture-MS Homo sapiens
24 RAB7A 7879
Proximity Label-MS Homo sapiens
25 PTPRH 5794
Proximity Label-MS Homo sapiens
26 MEA1  
Affinity Capture-MS Homo sapiens
27 KRAS 3845
Proximity Label-MS Homo sapiens
28 RHOB 388
Proximity Label-MS Homo sapiens
29 SGIP1  
Affinity Capture-MS Homo sapiens
30 CAV1 857
Proximity Label-MS Homo sapiens
31 DUSP23 54935
Proximity Label-MS Homo sapiens
32 STX6 10228
Proximity Label-MS Homo sapiens
33 NKIRAS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 RAB9A 9367
Proximity Label-MS Homo sapiens
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