Gene description for ZNF473
Gene name zinc finger protein 473
Gene symbol ZNF473
Other names/aliases HZFP100
ZN473
Species Homo sapiens
 Database cross references - ZNF473
ExoCarta ExoCarta_25888
Vesiclepedia VP_25888
Entrez Gene 25888
HGNC 23239
UniProt Q8WTR7  
 ZNF473 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for ZNF473
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IBA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    mRNA 3'-end processing by stem-loop binding and cleavage GO:0006398 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    Cajal body GO:0015030 IDA
 Experiment description of studies that identified ZNF473 in sEVs
1
Experiment ID 235
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for ZNF473
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF792 126375
Two-hybrid Homo sapiens
2 ZNF202  
Two-hybrid Homo sapiens
3 LSM10  
Affinity Capture-Western Homo sapiens
4 TRIM41 90933
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 CLK2 1196
Two-hybrid Homo sapiens
6 SLBP  
Two-hybrid Homo sapiens
7 GPATCH2L  
Two-hybrid Homo sapiens
8 CLK3  
Two-hybrid Homo sapiens
9 AEN  
Two-hybrid Homo sapiens
10 ZSCAN12  
Two-hybrid Homo sapiens
11 RNPS1 10921
Two-hybrid Homo sapiens
12 KRTAP10-10  
Two-hybrid Homo sapiens
13 ZNF408  
Two-hybrid Homo sapiens
14 PRPF31 26121
Two-hybrid Homo sapiens
15 DHX57 90957
Two-hybrid Homo sapiens
16 ZNF497  
Two-hybrid Homo sapiens
17 KRTAP10-8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 ZNF837  
Two-hybrid Homo sapiens
19 KRTAP10-9  
Two-hybrid Homo sapiens
20 LSM11  
Reconstituted Complex Homo sapiens
21 ZNF250  
Two-hybrid Homo sapiens
View the network image/svg+xml



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