Gene description for WIPI2
Gene name WD repeat domain, phosphoinositide interacting 2
Gene symbol WIPI2
Other names/aliases ATG18B
Atg21
CGI-50
WIPI-2
Species Homo sapiens
 Database cross references - WIPI2
ExoCarta ExoCarta_26100
Vesiclepedia VP_26100
Entrez Gene 26100
HGNC 32225
MIM 609225
UniProt Q9Y4P8  
 WIPI2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for WIPI2
Molecular Function
    protein binding GO:0005515 IPI
    phosphatidylinositol-5-phosphate binding GO:0010314 IDA
    phosphatidylinositol-3-phosphate binding GO:0032266 IBA
    phosphatidylinositol-3-phosphate binding GO:0032266 IDA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IBA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IDA
Biological Process
    autophagosome assembly GO:0000045 IDA
    autophagosome assembly GO:0000045 IMP
    autophagy of mitochondrion GO:0000422 IBA
    cellular response to starvation GO:0009267 IDA
    autophagy of peroxisome GO:0030242 IBA
    protein localization to phagophore assembly site GO:0034497 IBA
    protein localization to phagophore assembly site GO:0034497 IMP
    nucleophagy GO:0044804 IBA
    glycophagy GO:0061723 IBA
    protein lipidation involved in autophagosome assembly GO:0061739 IBA
    autophagosome maturation GO:0097352 IMP
    xenophagy GO:0098792 IEA
Subcellular Localization
    phagophore assembly site GO:0000407 IDA
    nucleoplasm GO:0005654 IDA
    autophagosome GO:0005776 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IDA
    protein-containing complex GO:0032991 IDA
    phagophore assembly site membrane GO:0034045 IBA
    phagophore assembly site membrane GO:0034045 IDA
 Experiment description of studies that identified WIPI2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for WIPI2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TXLNA 200081
Co-fractionation Homo sapiens
2 DPH2 1802
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 NAGK 55577
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 ATG16L1 55054
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
5 NPLOC4 55666
Affinity Capture-Western Homo sapiens
6 MRRF  
Two-hybrid Homo sapiens
7 DPH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 POT1  
Two-hybrid Homo sapiens
9 FBXL6  
Proximity Label-MS Homo sapiens
10 ATG5 9474
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 BTBD8  
Affinity Capture-MS Homo sapiens
12 C16orf70  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 PFKP 5214
Affinity Capture-MS Homo sapiens
14 BBS7 55212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 COPS6 10980
Two-hybrid Homo sapiens
16 ATG12  
Affinity Capture-Western Homo sapiens
17 ZFAND6  
Co-fractionation Homo sapiens
18 ATG101  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
19 EGFR 1956
Affinity Capture-MS Homo sapiens
20 TERF2IP 54386
Two-hybrid Homo sapiens
21 ARL6IP1 23204
Two-hybrid Homo sapiens
22 RABGAP1 23637
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PDPK1 5170
Affinity Capture-Western Homo sapiens
24 GCN1L1 10985
Affinity Capture-MS Homo sapiens
25 CUL4B 8450
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 SYP  
Two-hybrid Homo sapiens
27 FLNC 2318
Affinity Capture-MS Homo sapiens
28 BCAS3 54828
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 RABAC1 10567
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
30 RPA3 6119
Proximity Label-MS Homo sapiens
31 VCP 7415
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
32 RAB11A 8766
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
33 CUL4A 8451
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
34 COPS5 10987
Affinity Capture-MS Homo sapiens
35 DDB1 1642
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
36 DNM2 1785
Affinity Capture-MS Homo sapiens
37 DGAT2L6  
Two-hybrid Homo sapiens
38 BNIP3L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 DNAJB1 3337
Affinity Capture-MS Homo sapiens
40 NUDC 10726
Affinity Capture-MS Homo sapiens
41 TIMM13 26517
Co-fractionation Homo sapiens
42 REEP6  
Two-hybrid Homo sapiens
43 HUWE1 10075
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which WIPI2 is involved
PathwayEvidenceSource
Autophagy TAS Reactome
Macroautophagy TAS Reactome





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