Gene description for Grk6
Gene name G protein-coupled receptor kinase 6
Gene symbol Grk6
Other names/aliases Gprk6
Species Mus musculus
 Database cross references - Grk6
ExoCarta ExoCarta_26385
Vesiclepedia VP_26385
Entrez Gene 26385
UniProt O70293  
 Grk6 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Grk6
Molecular Function
    G protein-coupled receptor kinase activity GO:0004703 IBA
    G protein-coupled receptor kinase activity GO:0004703 IEA
    G protein-coupled receptor kinase activity GO:0004703 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    beta-adrenergic receptor kinase activity GO:0047696 IEA
    beta-adrenergic receptor kinase activity GO:0047696 ISO
Biological Process
    desensitization of G protein-coupled receptor signaling pathway GO:0002029 ISO
    regulation of signal transduction GO:0009966 IBA
    Wnt signaling pathway GO:0016055 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    membrane GO:0016020 IEA
 Experiment description of studies that identified Grk6 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Grk6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sclt1  
Co-fractionation Mus musculus
2 Prss23 76453
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Grk6 is involved
PathwayEvidenceSource
G alpha (s) signalling events IEA Reactome
GPCR downstream signalling IEA Reactome
Signal Transduction IEA Reactome
Signaling by GPCR IEA Reactome





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