Experiment description of studies that identified AMY1A in sEVs

1 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 66 MISEV standards ✔ IEM Biophysical techniques ✔ TSG101|Alix|CD63|CD81 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19199708     Organism Homo sapiens Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR" Journal name JPR Publication year 2009 Sample Saliva Sample name Saliva Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy

4 Experiment ID 902 MISEV standards ✔ EM Biophysical techniques ✔ CD63|CD9 Enriched markers ✔ AMY|ALB|CANX Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 33432243     Organism Homo sapiens Experiment description Exosome detection via the ultrafast-isolation system: EXODUS Authors "Chen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F." Journal name Nat Methods Publication year 2021 Sample Saliva Sample name Saliva Isolation/purification methods Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

5 Experiment ID 903 MISEV standards ✔ EM Biophysical techniques ✔ CD63|CD9 Enriched markers ✔ AMY|ALB|CANX Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 33432243     Organism Homo sapiens Experiment description Exosome detection via the ultrafast-isolation system: EXODUS Authors "Chen Y, Zhu Q, Cheng L, Wang Y, Li M, Yang Q, Hu L, Lou D, Li J, Dong X, Lee LP, Liu F." Journal name Nat Methods Publication year 2021 Sample Saliva Sample name Saliva Isolation/purification methods Filtration Polyethylene glycol-based precipitation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting

Protein-protein interactions for AMY1A

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PLK4   Affinity Capture-MS Homo sapiens 2 SMYD3 64754 Affinity Capture-MS Homo sapiens 3 RASSF6   Affinity Capture-MS Homo sapiens 4 AKAP8   Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens 5 KRAS 3845 Negative Genetic Homo sapiens 6 SMARCB1 6598 Affinity Capture-MS Homo sapiens 7 EGFR 1956 Negative Genetic Homo sapiens 8 AKAP1 8165 Affinity Capture-Western Homo sapiens 9 MUC5B   Co-purification Homo sapiens 10 CDK2AP1   Affinity Capture-MS Homo sapiens View the network

Pathways in which AMY1A is involved

No pathways found