Gene description for SMYD3
Gene name SET and MYND domain containing 3
Gene symbol SMYD3
Other names/aliases KMT3E
ZMYND1
ZNFN3A1
bA74P14.1
Species Homo sapiens
 Database cross references - SMYD3
ExoCarta ExoCarta_64754
Vesiclepedia VP_64754
Entrez Gene 64754
HGNC 15513
MIM 608783
UniProt Q9H7B4  
 SMYD3 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SMYD3
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IEA
    RNA polymerase II complex binding GO:0000993 IEA
    RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding GO:0001162 IEA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    histone H4 methyltransferase activity GO:0140939 IMP
    histone H3K36 dimethyltransferase activity GO:0140954 ISS
    histone H3K4 trimethyltransferase activity GO:0140999 IEA
Biological Process
    nucleosome assembly GO:0006334 IEA
    myotube cell development GO:0014904 IEA
    methylation GO:0032259 IEA
    establishment of protein localization GO:0045184 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    cellular response to dexamethasone stimulus GO:0071549 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IDA
 Experiment description of studies that identified SMYD3 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SMYD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MRPL41 64975
Affinity Capture-MS Homo sapiens
2 APP 351
Reconstituted Complex Homo sapiens
3 NDUFAB1 4706
Two-hybrid Homo sapiens
4 NUP54 53371
Two-hybrid Homo sapiens
5 SNX27 81609
Affinity Capture-MS Homo sapiens
6 ALDH3A2 224
Affinity Capture-MS Homo sapiens
7 PLCB3 5331
Co-fractionation Homo sapiens
8 AMY1A 276
Affinity Capture-MS Homo sapiens
9 MRPL47 57129
Affinity Capture-MS Homo sapiens
10 NTMT1 28989
Affinity Capture-MS Homo sapiens
11 RPL7A 6130
Affinity Capture-MS Homo sapiens
12 TP53 7157
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
13 CSTF2T  
Two-hybrid Homo sapiens
14 HIST1H4I 8294
Affinity Capture-MS Homo sapiens
15 MEST 4232
Affinity Capture-MS Homo sapiens
16 RCAN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 RPL27 6155
Affinity Capture-MS Homo sapiens
18 HSP90AB4P 664618
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 CDC37 11140
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
20 HSP90AA5P 730211
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SMYD1  
Affinity Capture-MS Homo sapiens
22 MRPL24  
Affinity Capture-MS Homo sapiens
23 MKRN3  
Two-hybrid Homo sapiens
24 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 ZNF557  
Two-hybrid Homo sapiens
26 PTGER3  
Affinity Capture-MS Homo sapiens
27 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 METTL21B  
Affinity Capture-MS Homo sapiens
29 POLR2A 5430
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 UBE2R2 54926
Affinity Capture-Western Homo sapiens
31 HIST3H3 8290
Biochemical Activity Homo sapiens
32 ESR1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
33 HSP90AB3P 3327
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 KDM3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 MAGED4B  
Affinity Capture-MS Homo sapiens
36 NFYB  
Two-hybrid Homo sapiens
37 EZH2  
Protein-RNA Homo sapiens
Protein-RNA Homo sapiens
38 NME1 4830
Affinity Capture-MS Homo sapiens
39 HELZ 9931
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
40 KMT2E  
Affinity Capture-MS Homo sapiens
41 H3F3C 440093
Two-hybrid Homo sapiens
42 MRPL23 6150
Affinity Capture-MS Homo sapiens
43 RPS27L 51065
Affinity Capture-MS Homo sapiens
44 PKN1 5585
Two-hybrid Homo sapiens
45 CYP39A1  
Two-hybrid Homo sapiens
46 PTGES3 10728
Affinity Capture-MS Homo sapiens
47 TUBA1B 10376
Affinity Capture-MS Homo sapiens
48 SHC3  
Two-hybrid Homo sapiens
49 VANGL1 81839
Two-hybrid Homo sapiens
50 TRMT2A 27037
Co-fractionation Homo sapiens
51 MRPL28 10573
Affinity Capture-MS Homo sapiens
52 RPLP2 6181
Affinity Capture-MS Homo sapiens
53 ENPP1 5167
Affinity Capture-MS Homo sapiens
54 GYG2  
Affinity Capture-MS Homo sapiens
55 CAMK2B 816
Two-hybrid Homo sapiens
56 TRIT1  
Two-hybrid Homo sapiens
57 EED  
Protein-RNA Homo sapiens
View the network image/svg+xml
 Pathways in which SMYD3 is involved
PathwayEvidenceSource
Chromatin modifying enzymes TAS Reactome
Chromatin organization TAS Reactome
PKMTs methylate histone lysines TAS Reactome





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