Gene description for GPT
Gene name glutamic-pyruvate transaminase (alanine aminotransferase)
Gene symbol GPT
Other names/aliases AAT1
ALT1
GPT1
Species Homo sapiens
 Database cross references - GPT
ExoCarta ExoCarta_2875
Vesiclepedia VP_2875
Entrez Gene 2875
HGNC 4552
MIM 138200
UniProt P24298  
 GPT identified in sEVs derived from the following tissue/cell type
Urine 19056867    
 Gene ontology annotations for GPT
Molecular Function
    L-alanine:2-oxoglutarate aminotransferase activity GO:0004021 NAS
    pyridoxal phosphate binding GO:0030170 IEA
Biological Process
    biosynthetic process GO:0009058 IEA
    cellular response to insulin stimulus GO:0032869 IEA
    response to starvation GO:0042594 IEA
    L-alanine catabolic process GO:0042853 IEA
    positive regulation of gluconeogenesis GO:0045722 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified GPT in sEVs
1
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for GPT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SASS6 163786
Affinity Capture-MS Homo sapiens
2 CFL1 1072
Co-fractionation Homo sapiens
3 CDC7  
Two-hybrid Homo sapiens
4 BRD2  
Affinity Capture-MS Homo sapiens
5 GPT2  
Affinity Capture-MS Homo sapiens
6 KPNA1 3836
Affinity Capture-MS Homo sapiens
7 CUL4A 8451
Affinity Capture-MS Homo sapiens
8 MAEA 10296
Affinity Capture-MS Homo sapiens
9 GPRC5D  
Affinity Capture-MS Homo sapiens
10 RALA 5898
Affinity Capture-MS Homo sapiens
11 PLD1 5337
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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