Gene description for ATAD2
Gene name ATPase family, AAA domain containing 2
Gene symbol ATAD2
Other names/aliases ANCCA
CT137
PRO2000
Species Homo sapiens
 Database cross references - ATAD2
ExoCarta ExoCarta_29028
Vesiclepedia VP_29028
Entrez Gene 29028
HGNC 30123
MIM 611941
UniProt Q6PL18  
 ATAD2 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Urine 19056867    
 Gene ontology annotations for ATAD2
Molecular Function
    chromatin binding GO:0003682 IBA
    DNA clamp loader activity GO:0003689 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 IDA
    histone binding GO:0042393 IBA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    nucleosome assembly GO:0006334 IBA
    nucleosome disassembly GO:0006337 IBA
    transcription initiation-coupled chromatin remodeling GO:0045815 IBA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    chromatin looping GO:0140588 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ATAD2 in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ATAD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
2 TRIM25 7706
Affinity Capture-Western Homo sapiens
3 NUMA1 4926
Affinity Capture-MS Homo sapiens
4 FASN 2194
Negative Genetic Homo sapiens
5 CSNK2B 1460
Affinity Capture-MS Homo sapiens
6 PARP1 142
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
7 CENPA  
Proximity Label-MS Homo sapiens
8 FANCD2  
Affinity Capture-MS Homo sapiens
9 HIST2H4A 8370
Reconstituted Complex Homo sapiens
10 SPTBN2 6712
Affinity Capture-MS Homo sapiens
11 SIRT7  
Affinity Capture-MS Homo sapiens
12 E2F4  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
13 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
14 POLE3  
Co-fractionation Homo sapiens
15 HMGN5 79366
Affinity Capture-MS Homo sapiens
16 CBX3 11335
Proximity Label-MS Homo sapiens
17 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
18 HIST3H3 8290
Reconstituted Complex Homo sapiens
19 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
20 NXF1 10482
Affinity Capture-RNA Homo sapiens
21 ESR1  
Affinity Capture-Western Homo sapiens
22 E2F3  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
23 E2F2 1870
Reconstituted Complex Homo sapiens
24 WDR76  
Affinity Capture-MS Homo sapiens
25 MYC  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
26 Naa50  
Affinity Capture-MS Mus musculus
27 E2F1 1869
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
28 NCOA3  
Affinity Capture-Western Homo sapiens
29 HIST1H3A 8350
Proximity Label-MS Homo sapiens
30 BRD3 8019
Affinity Capture-MS Homo sapiens
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