Gene description for ALG5
Gene name ALG5, dolichyl-phosphate beta-glucosyltransferase
Gene symbol ALG5
Other names/aliases bA421P11.2
Species Homo sapiens
 Database cross references - ALG5
ExoCarta ExoCarta_29880
Vesiclepedia VP_29880
Entrez Gene 29880
HGNC 20266
MIM 604565
UniProt Q9Y673  
 ALG5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ALG5
Molecular Function
    dolichyl-phosphate beta-glucosyltransferase activity GO:0004581 IGI
    dolichyl-phosphate beta-glucosyltransferase activity GO:0004581 TAS
    protein binding GO:0005515 IPI
Biological Process
    protein N-linked glycosylation GO:0006487 IBA
    protein N-linked glycosylation GO:0006487 IMP
    dolichol-linked oligosaccharide biosynthetic process GO:0006488 IMP
    determination of left/right symmetry GO:0007368 IEA
    protein N-linked glycosylation via asparagine GO:0018279 TAS
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
    cytoplasmic side of rough endoplasmic reticulum membrane GO:0098556 IGI
 Experiment description of studies that identified ALG5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ALG5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HFE  
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 HIST1H2BL 8340
Cross-Linking-MS (XL-MS) Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 PCDHGB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SLC1A5 6510
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TMEM9 252839
Affinity Capture-MS Homo sapiens
8 TMEM59 9528
Affinity Capture-MS Homo sapiens
9 HIST1H4A 8359
Cross-Linking-MS (XL-MS) Homo sapiens
10 LDLRAD1  
Affinity Capture-MS Homo sapiens
11 TNFSF8  
Affinity Capture-MS Homo sapiens
12 ADCY9 115
Affinity Capture-MS Homo sapiens
13 DLK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 LAMP1 3916
Proximity Label-MS Homo sapiens
15 CD1B 910
Affinity Capture-MS Homo sapiens
16 HIST1H2BH 8345
Cross-Linking-MS (XL-MS) Homo sapiens
17 LMNA 4000
Proximity Label-MS Homo sapiens
18 TSPAN15 23555
Affinity Capture-MS Homo sapiens
19 C9orf78 51759
Affinity Capture-MS Homo sapiens
20 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
21 EMP3 2014
Affinity Capture-MS Homo sapiens
22 PSEN2 5664
Affinity Capture-MS Homo sapiens
23 LRRC25  
Affinity Capture-MS Homo sapiens
24 IL13RA2 3598
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 EDA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 PLD3 23646
Affinity Capture-MS Homo sapiens
27 LSMEM2  
Affinity Capture-MS Homo sapiens
28 RPA3 6119
Proximity Label-MS Homo sapiens
29 HIST1H2BA 255626
Cross-Linking-MS (XL-MS) Homo sapiens
30 POMK  
Affinity Capture-MS Homo sapiens
31 PKD2 5311
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PVR 5817
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 TMEM63A 9725
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CACNG1  
Affinity Capture-MS Homo sapiens
35 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
36 SPINT2 10653
Affinity Capture-MS Homo sapiens
37 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
38 H2AFY 9555
Cross-Linking-MS (XL-MS) Homo sapiens
39 ESYT1 23344
Cross-Linking-MS (XL-MS) Homo sapiens
40 PAICS 10606
Cross-Linking-MS (XL-MS) Homo sapiens
41 CACNG4  
Affinity Capture-MS Homo sapiens
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