Gene description for ICAM2
Gene name intercellular adhesion molecule 2
Gene symbol ICAM2
Other names/aliases CD102
Species Homo sapiens
 Database cross references - ICAM2
ExoCarta ExoCarta_3384
Vesiclepedia VP_3384
Entrez Gene 3384
HGNC 5345
MIM 146630
UniProt P13598  
 ICAM2 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for ICAM2
Molecular Function
    integrin binding GO:0005178 IBA
    integrin binding GO:0005178 IPI
Biological Process
    cell adhesion GO:0007155 IBA
    cell-cell adhesion GO:0098609 IEA
Subcellular Localization
    uropod GO:0001931 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 TAS
    microvillus GO:0005902 ISS
    membrane GO:0016020 HDA
    cleavage furrow GO:0032154 IDA
 Experiment description of studies that identified ICAM2 in sEVs
1
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ICAM2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB18 22931
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 F2RL1  
Two-hybrid Homo sapiens
3 LRFN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NRP1 8829
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 HBD 3045
Affinity Capture-MS Homo sapiens
6 CNNM4 26504
Affinity Capture-MS Homo sapiens
7 MAD2L2 10459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SACM1L 22908
Affinity Capture-MS Homo sapiens
9 ATP1A3 478
Affinity Capture-MS Homo sapiens
10 LMAN2L 81562
Affinity Capture-MS Homo sapiens
11 PCDH1 5097
Affinity Capture-MS Homo sapiens
12 CMTM8  
Affinity Capture-MS Homo sapiens
13 CTPS2 56474
Affinity Capture-MS Homo sapiens
14 TM9SF3 56889
Affinity Capture-MS Homo sapiens
15 ARL6IP5 10550
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ATP13A2  
Two-hybrid Homo sapiens
17 RAB38 23682
Affinity Capture-MS Homo sapiens
18 EZR 7430
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
19 RSU1 6251
Affinity Capture-MS Homo sapiens
20 TUBB8 347688
Affinity Capture-MS Homo sapiens
21 CYP2S1  
Affinity Capture-MS Homo sapiens
22 BSCL2  
Affinity Capture-MS Homo sapiens
23 RAB29 8934
Affinity Capture-MS Homo sapiens
24 RTN2 6253
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 LGALS1 3956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 FUT8 2530
Affinity Capture-MS Homo sapiens
27 MFSD3  
Affinity Capture-MS Homo sapiens
28 TUBA1A 7846
Affinity Capture-MS Homo sapiens
29 UBE3C 9690
Affinity Capture-MS Homo sapiens
30 TEX264 51368
Affinity Capture-MS Homo sapiens
31 TGFBR1 7046
Affinity Capture-MS Homo sapiens
32 SLC35E2B  
Affinity Capture-MS Homo sapiens
33 ITFG1 81533
Affinity Capture-MS Homo sapiens
34 RDX 5962
Co-crystal Structure Homo sapiens
35 DAGLB 221955
Affinity Capture-MS Homo sapiens
36 IPO13 9670
Affinity Capture-MS Homo sapiens
37 C1QL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 ACVR1B 91
Affinity Capture-MS Homo sapiens
39 HFE  
Affinity Capture-MS Homo sapiens
40 LRRC8E 80131
Affinity Capture-MS Homo sapiens
41 RAB3B 5865
Affinity Capture-MS Homo sapiens
42 TM9SF4 9777
Affinity Capture-MS Homo sapiens
43 RPN1 6184
Affinity Capture-MS Homo sapiens
44 SLC20A1 6574
Affinity Capture-MS Homo sapiens
45 TM9SF1 10548
Affinity Capture-MS Homo sapiens
46 MSN 4478
Reconstituted Complex Homo sapiens
47 C16orf58 64755
Affinity Capture-MS Homo sapiens
48 CNNM2 54805
Affinity Capture-MS Homo sapiens
49 ATP2C1 27032
Affinity Capture-MS Homo sapiens
50 DAD1 1603
Affinity Capture-MS Homo sapiens
51 SLC25A19  
Affinity Capture-MS Homo sapiens
52 ITM2B 9445
Affinity Capture-MS Homo sapiens
53 TNFSF9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 YIF1B 90522
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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