Gene description for ITGAM
Gene name integrin, alpha M (complement component 3 receptor 3 subunit)
Gene symbol ITGAM
Other names/aliases CD11B
CR3A
MAC-1
MAC1A
MO1A
SLEB6
Species Homo sapiens
 Database cross references - ITGAM
ExoCarta ExoCarta_3684
Vesiclepedia VP_3684
Entrez Gene 3684
HGNC 6149
MIM 120980
UniProt P11215  
 ITGAM identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for ITGAM
Molecular Function
    amyloid-beta binding GO:0001540 IC
    complement component C3b binding GO:0001851 ISS
    integrin binding GO:0005178 IBA
    integrin binding GO:0005178 NAS
    protein binding GO:0005515 IPI
    heat shock protein binding GO:0031072 IPI
    cargo receptor activity GO:0038024 ISS
    cargo receptor activity GO:0038024 ISS
    metal ion binding GO:0046872 IEA
Biological Process
    microglial cell activation GO:0001774 ISS
    complement receptor mediated signaling pathway GO:0002430 ISS
    response to ischemia GO:0002931 IEA
    receptor-mediated endocytosis GO:0006898 ISS
    phagocytosis, engulfment GO:0006911 ISS
    cell adhesion GO:0007155 TAS
    cell-matrix adhesion GO:0007160 IBA
    cell-matrix adhesion GO:0007160 IDA
    integrin-mediated signaling pathway GO:0007229 IBA
    integrin-mediated signaling pathway GO:0007229 NAS
    response to mechanical stimulus GO:0009612 IEA
    ectodermal cell differentiation GO:0010668 IEP
    forebrain development GO:0030900 ISS
    response to estradiol GO:0032355 IEA
    positive regulation of superoxide anion generation GO:0032930 IGI
    positive regulation of superoxide anion generation GO:0032930 ISS
    cell adhesion mediated by integrin GO:0033627 IBA
    heterotypic cell-cell adhesion GO:0034113 IEA
    positive regulation of neutrophil degranulation GO:0043315 IGI
    innate immune response GO:0045087 IEA
    negative regulation of dopamine metabolic process GO:0045963 ISS
    positive regulation of protein targeting to membrane GO:0090314 ISS
    amyloid-beta clearance GO:0097242 ISS
    cell-cell adhesion GO:0098609 IBA
    cell-cell adhesion via plasma-membrane adhesion molecules GO:0098742 NAS
    response to Gram-positive bacterium GO:0140459 IEA
    complement-mediated synapse pruning GO:0150062 ISS
    vertebrate eye-specific patterning GO:0150064 ISS
    positive regulation of microglial cell mediated cytotoxicity GO:1904151 ISS
    response to curcumin GO:1904643 IEA
    positive regulation of prostaglandin-E synthase activity GO:2000363 ISS
Subcellular Localization
    extracellular space GO:0005615 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    integrin complex GO:0008305 IBA
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 NAS
    cell surface GO:0009986 HDA
    cell surface GO:0009986 IDA
    integrin alphaM-beta2 complex GO:0034688 IPI
    integrin alphaM-beta2 complex GO:0034688 ISS
    specific granule membrane GO:0035579 TAS
    plasma membrane raft GO:0044853 IDA
    extracellular exosome GO:0070062 HDA
    tertiary granule membrane GO:0070821 TAS
 Experiment description of studies that identified ITGAM in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ITGAM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TMEM52B 120939
Two-hybrid Homo sapiens
2 ABHD16A 7920
Two-hybrid Homo sapiens
3 PRTN3 5657
Affinity Capture-Western Homo sapiens
4 KIR2DL3  
Two-hybrid Homo sapiens
5 RHBDD1 84236
Affinity Capture-MS Homo sapiens
6 HSD17B13  
Two-hybrid Homo sapiens
7 DKK4  
Two-hybrid Homo sapiens
8 SLC10A6  
Two-hybrid Homo sapiens
9 TMEM14B  
Two-hybrid Homo sapiens
10 AGPAT1 10554
Affinity Capture-MS Homo sapiens
11 ICAM1 3383
Reconstituted Complex Homo sapiens
12 PIGP  
Two-hybrid Homo sapiens
13 CLRN1  
Two-hybrid Homo sapiens
14 PLAUR 5329
Reconstituted Complex Homo sapiens
15 GNG8  
Affinity Capture-MS Homo sapiens
16 ERGIC3 51614
Two-hybrid Homo sapiens
17 BCAP31 10134
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
18 IRAK1  
Affinity Capture-Western Homo sapiens
19 REEP4  
Two-hybrid Homo sapiens
20 GJA8  
Two-hybrid Homo sapiens
21 REEP2  
Two-hybrid Homo sapiens
22 MRM1  
Two-hybrid Homo sapiens
23 DDX58 23586
Affinity Capture-RNA Homo sapiens
24 CREB3L1  
Two-hybrid Homo sapiens
25 MMGT1 93380
Two-hybrid Homo sapiens
26 AUP1 550
Two-hybrid Homo sapiens
27 GORAB 92344
Two-hybrid Homo sapiens
28 JAM3 83700
Reconstituted Complex Homo sapiens
29 CCDC155  
Two-hybrid Homo sapiens
30 GPR42  
Two-hybrid Homo sapiens
31 CD79A  
Two-hybrid Homo sapiens
32 KLK10 5655
Affinity Capture-MS Homo sapiens
33 C16orf58 64755
Two-hybrid Homo sapiens
34 RAD21 5885
Affinity Capture-Western Homo sapiens
35 MUC1 4582
Two-hybrid Homo sapiens
36 SHARPIN  
Protein-peptide Homo sapiens
37 GJB1 2705
Two-hybrid Homo sapiens
38 KCNJ6  
Two-hybrid Homo sapiens
39 TMEM229B  
Two-hybrid Homo sapiens
40 TMEM31  
Two-hybrid Homo sapiens
41 ARL13B 200894
Two-hybrid Homo sapiens
42 SYT2  
Two-hybrid Homo sapiens
43 SCN3B  
Two-hybrid Homo sapiens
44 ELOVL4  
Two-hybrid Homo sapiens
45 PGRMC2 10424
Two-hybrid Homo sapiens
46 MGST3 4259
Two-hybrid Homo sapiens
47 TMX2 51075
Two-hybrid Homo sapiens
View the network image/svg+xml



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