Gene description for GJB1
Gene name gap junction protein, beta 1, 32kDa
Gene symbol GJB1
Other names/aliases CMTX
CMTX1
CX32
Species Homo sapiens
 Database cross references - GJB1
ExoCarta ExoCarta_2705
Vesiclepedia VP_2705
Entrez Gene 2705
HGNC 4283
MIM 304040
UniProt P08034  
 GJB1 identified in sEVs derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
 Gene ontology annotations for GJB1
Molecular Function
    gap junction channel activity GO:0005243 IBA
    gap junction channel activity GO:0005243 TAS
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IEA
Biological Process
    cell-cell signaling GO:0007267 IBA
    nervous system development GO:0007399 TAS
    purine ribonucleotide transport GO:0015868 IEA
    gap junction assembly GO:0016264 TAS
    transmembrane transport GO:0055085 IEA
    epididymis development GO:1905867 IEA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
    connexin complex GO:0005922 IBA
    lateral plasma membrane GO:0016328 IEA
 Experiment description of studies that identified GJB1 in sEVs
1
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for GJB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDIPT 10423
Two-hybrid Homo sapiens
2 HNRNPH1 3187
Affinity Capture-MS Homo sapiens
3 C2CD2L 9854
Two-hybrid Homo sapiens
4 C1GALT1 56913
Two-hybrid Homo sapiens
5 OGDHL  
Affinity Capture-MS Homo sapiens
6 CANX 821
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
7 TMEM14C 51522
Two-hybrid Homo sapiens
8 TMEM19  
Two-hybrid Homo sapiens
9 LAT 27040
Two-hybrid Homo sapiens
10 GJB2  
Two-hybrid Homo sapiens
11 TM4SF4  
Two-hybrid Homo sapiens
12 SLC12A7 10723
Two-hybrid Homo sapiens
13 BMP10  
Two-hybrid Homo sapiens
14 OCLN 100506658
Affinity Capture-Western Homo sapiens
15 TMEM218  
Two-hybrid Homo sapiens
16 JUP 3728
Affinity Capture-Western Homo sapiens
17 MRPL30  
Affinity Capture-MS Homo sapiens
18 EMC4 51234
Affinity Capture-Western Homo sapiens
19 STX8 9482
Two-hybrid Homo sapiens
20 YIPF6 286451
Two-hybrid Homo sapiens
21 RTP2  
Two-hybrid Homo sapiens
22 IER3IP1 51124
Two-hybrid Homo sapiens
23 MAML1  
Affinity Capture-MS Homo sapiens
24 MAL2 114569
Two-hybrid Homo sapiens
25 EMC10 284361
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
26 GPR25  
Two-hybrid Homo sapiens
27 CMTM3 123920
Two-hybrid Homo sapiens
28 VEGFA 7422
Two-hybrid Homo sapiens
29 MS4A13  
Two-hybrid Homo sapiens
30 CLEC7A  
Two-hybrid Homo sapiens
31 CD79A  
Two-hybrid Homo sapiens
32 EFNA5  
Two-hybrid Homo sapiens
33 EMP3 2014
Two-hybrid Homo sapiens
34 NIPAL4  
Two-hybrid Homo sapiens
35 APP 351
Reconstituted Complex Homo sapiens
36 MAL  
Two-hybrid Homo sapiens
37 HSPA5 3309
Affinity Capture-Western Homo sapiens
38 TEX264 51368
Two-hybrid Homo sapiens
39 APOL3  
Two-hybrid Homo sapiens
40 CORO1B 57175
Affinity Capture-MS Homo sapiens
41 CNST  
Reconstituted Complex Homo sapiens
42 OGDH 4967
Affinity Capture-MS Homo sapiens
43 CD37 951
Two-hybrid Homo sapiens
44 MSI2 124540
Affinity Capture-MS Homo sapiens
45 CMTM5  
Two-hybrid Homo sapiens
46 DLST 1743
Affinity Capture-MS Homo sapiens
47 PTPN9 5780
Two-hybrid Homo sapiens
48 UBQLN4 56893
Affinity Capture-Western Homo sapiens
49 CTXN3  
Two-hybrid Homo sapiens
50 VKORC1 79001
Two-hybrid Homo sapiens
51 MRPS36 92259
Affinity Capture-MS Homo sapiens
52 CXCL9  
Two-hybrid Homo sapiens
53 NDUFB11 54539
Two-hybrid Homo sapiens
54 SERTM1  
Two-hybrid Homo sapiens
55 LHFPL2 10184
Two-hybrid Homo sapiens
56 CAV1 857
Affinity Capture-Western Homo sapiens
57 MFF 56947
Two-hybrid Homo sapiens
58 C3orf52  
Two-hybrid Homo sapiens
59 ITGAM 3684
Two-hybrid Homo sapiens
60 DNAJB11 51726
Affinity Capture-MS Homo sapiens
61 TMEM120B  
Two-hybrid Homo sapiens
62 UPK1B 7348
Two-hybrid Homo sapiens
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