Gene description for ARHGAP4
Gene name Rho GTPase activating protein 4
Gene symbol ARHGAP4
Other names/aliases C1
RGC1
RhoGAP4
SrGAP4
p115
Species Homo sapiens
 Database cross references - ARHGAP4
ExoCarta ExoCarta_393
Vesiclepedia VP_393
Entrez Gene 393
HGNC 674
MIM 300023
UniProt P98171  
 ARHGAP4 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for ARHGAP4
Molecular Function
    GTPase activator activity GO:0005096 TAS
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    cytoskeleton organization GO:0007010 TAS
    Rho protein signal transduction GO:0007266 TAS
    nervous system development GO:0007399 IEA
    negative regulation of fibroblast migration GO:0010764 IEA
    negative regulation of cell migration GO:0030336 IBA
    negative regulation of axon extension GO:0030517 IEA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IEA
    growth cone GO:0030426 IEA
 Experiment description of studies that identified ARHGAP4 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARHGAP4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MYCBP 26292
Affinity Capture-MS Homo sapiens
2 SCARNA22  
Affinity Capture-RNA Homo sapiens
3 RPS29 6235
Affinity Capture-MS Homo sapiens
4 RANBP9 10048
Affinity Capture-MS Homo sapiens
5 RAC1 5879
FRET Homo sapiens
6 WDR33 55339
Affinity Capture-MS Homo sapiens
7 ZNF252P-AS1  
Affinity Capture-MS Homo sapiens
8 FMNL1 752
Affinity Capture-MS Homo sapiens
9 ANKFY1 51479
Affinity Capture-MS Homo sapiens
10 NPAS1  
Affinity Capture-MS Homo sapiens
11 CAMSAP3  
Affinity Capture-MS Homo sapiens
12 SMARCA2 6595
Affinity Capture-MS Homo sapiens
13 BTF3 689
Affinity Capture-MS Homo sapiens
14 ARHGAP4 393
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
15 PCBP1 5093
Co-fractionation Homo sapiens
16 MAP3K1 4214
Two-hybrid Homo sapiens
17 FAM167A  
Affinity Capture-MS Homo sapiens
18 CSTA 1475
Affinity Capture-MS Homo sapiens
19 S100A9 6280
Affinity Capture-MS Homo sapiens
20 SMARCC2 6601
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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