Experiment description of studies that identified MAGEA4 in sEVs

1 Experiment ID 254 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1|CD81 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name MNT-1 Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 255 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name G1 Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 256 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name 501mel Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 257 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name Daju Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 258 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1|CD81 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name SKMEL28 Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

6 Experiment ID 259 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name A375M Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for MAGEA4

Protein Interactor ExoCarta ID Identification method PubMed Species 1 MAGEA9B   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 2 MAGEA8   Affinity Capture-MS Homo sapiens 3 ZNF576   Two-hybrid Homo sapiens 4 WBP2 23558 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 5 ARNT2   Two-hybrid Homo sapiens 6 RPP30 10556 Affinity Capture-MS Homo sapiens 7 TK1 7083 Two-hybrid Homo sapiens 8 POT1   Two-hybrid Homo sapiens 9 PPP1R13L 10848 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 HECTD4 283450 Affinity Capture-MS Homo sapiens 11 FXR1 8087 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 12 AMOTL2 51421 Two-hybrid Homo sapiens 13 TEKT4   Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 14 GTF3C1   Two-hybrid Homo sapiens 15 HDHD1   Two-hybrid Homo sapiens 16 BEX2   Two-hybrid Homo sapiens 17 HDDC3   Two-hybrid Homo sapiens 18 UQCRB 7381 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 19 COX8A   Two-hybrid Homo sapiens 20 MAGEB16   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 21 PSMD10 5716 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 22 RPL7L1 285855 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 23 APP 351 Reconstituted Complex Homo sapiens 24 TCEAL8   Affinity Capture-MS Homo sapiens 25 AMOTL1 154810 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 26 MAGEA10   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 DCAF6 55827 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 28 UVSSA   Two-hybrid Homo sapiens 29 LDLRAP1 26119 Two-hybrid Homo sapiens 30 FANK1   Affinity Capture-MS Homo sapiens 31 TSPYL1 7259 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 32 TSPYL2   Affinity Capture-MS Homo sapiens 33 TANGO6 79613 Affinity Capture-MS Homo sapiens 34 TIGD5   Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 35 CCDC85B   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 36 RAD18   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 37 MDM1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 38 TRIM69 140691 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 39 FNDC3A 22862 Affinity Capture-MS Homo sapiens 40 AMOT 154796 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 41 HLA-A 3105 Protein-peptide Homo sapiens Co-crystal Structure Homo sapiens 42 TSPYL4 23270 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 43 UBXN6 80700 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 44 IQGAP1 8826 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which MAGEA4 is involved

No pathways found