Gene description for NDUFS5
Gene name NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase)
Gene symbol NDUFS5
Other names/aliases CI-15k
CI15K
Species Homo sapiens
 Database cross references - NDUFS5
ExoCarta ExoCarta_4725
Vesiclepedia VP_4725
Entrez Gene 4725
HGNC 7712
MIM 603847
UniProt O43920  
 NDUFS5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for NDUFS5
Molecular Function
    protein binding GO:0005515 IPI
    NADH dehydrogenase (ubiquinone) activity GO:0008137 NAS
Biological Process
    mitochondrial electron transport, NADH to ubiquinone GO:0006120 NAS
    aerobic respiration GO:0009060 NAS
    mitochondrial respiratory chain complex I assembly GO:0032981 IBA
    mitochondrial respiratory chain complex I assembly GO:0032981 IMP
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 NAS
    proton transmembrane transport GO:1902600 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 TAS
    mitochondrial intermembrane space GO:0005758 IEA
    respiratory chain complex I GO:0045271 IBA
    respiratory chain complex I GO:0045271 IDA
    respiratory chain complex I GO:0045271 NAS
 Experiment description of studies that identified NDUFS5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for NDUFS5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CAPNS2 84290
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 Tmed2 56334
Affinity Capture-MS Mus musculus
4 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
5 SERPINB4 6318
Affinity Capture-MS Homo sapiens
6 NDUFS1 4719
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
7 LAP3 51056
Affinity Capture-MS Homo sapiens
8 POF1B 79983
Affinity Capture-MS Homo sapiens
9 NDUFB5 4711
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 NDUFA12 55967
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 MANF 7873
Affinity Capture-MS Homo sapiens
12 NDUFA4 4697
Affinity Capture-MS Homo sapiens
13 SMPDL3B 27293
Affinity Capture-MS Homo sapiens
14 CASP14 23581
Affinity Capture-MS Homo sapiens
15 NDUFS2 4720
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
16 CCDC90B  
Proximity Label-MS Homo sapiens
17 TOMM22 56993
Co-fractionation Homo sapiens
18 CHCHD4  
Affinity Capture-MS Homo sapiens
19 NDUFB9 4715
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
20 FOXRED1  
Affinity Capture-MS Homo sapiens
21 HEATR3 55027
Affinity Capture-MS Homo sapiens
22 NDUFS3 4722
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
23 OGDH 4967
Co-fractionation Homo sapiens
24 BCL7B  
Affinity Capture-MS Homo sapiens
25 NDUFB8 4714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 COX7A2L 9167
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
27 NDUFA7 4701
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TIMMDC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 NDUFA5 4698
Affinity Capture-MS Homo sapiens
30 ARMCX3 51566
Co-fractionation Homo sapiens
31 SURF1  
Proximity Label-MS Homo sapiens
32 C20orf24  
Co-fractionation Homo sapiens
33 CPA4 51200
Affinity Capture-MS Homo sapiens
34 KPRP 448834
Affinity Capture-MS Homo sapiens
35 AIFM1 9131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 NDUFAF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 PHB 5245
Co-fractionation Homo sapiens
38 TOMM40 10452
Co-fractionation Homo sapiens
39 NDUFV3 4731
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 MECP2 4204
Affinity Capture-MS Homo sapiens
41 MYCN  
Affinity Capture-MS Homo sapiens
42 TMEM132D  
Two-hybrid Homo sapiens
43 NDUFA6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 VAPA 9218
Affinity Capture-MS Homo sapiens
45 UQCRQ 27089
Co-fractionation Homo sapiens
46 NDUFA1 4694
Affinity Capture-MS Homo sapiens
47 ALOXE3  
Affinity Capture-MS Homo sapiens
48 NDUFB1 4707
Affinity Capture-MS Homo sapiens
49 WBP4  
Affinity Capture-MS Homo sapiens
50 MTCH2 23788
Co-fractionation Homo sapiens
51 NDUFS4 4724
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 NDUFB7 4713
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 SBSN 374897
Affinity Capture-MS Homo sapiens
54 HSP90B1 7184
Co-fractionation Homo sapiens
55 NDUFA8 4702
Co-fractionation Homo sapiens
56 DIABLO 56616
Proximity Label-MS Homo sapiens
57 MYH9 4627
Affinity Capture-MS Homo sapiens
58 ND1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 SERPINB8 5271
Affinity Capture-MS Homo sapiens
60 TMEM126B 55863
Affinity Capture-MS Homo sapiens
61 CYC1 1537
Co-fractionation Homo sapiens
62 UQCRC1 7384
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
63 ACTB 60
Co-fractionation Homo sapiens
64 TGM1 7051
Affinity Capture-MS Homo sapiens
65 DNAJC30  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 MYL12A 10627
Co-fractionation Homo sapiens
67 TMEM70  
Affinity Capture-MS Homo sapiens
68 NDUFAF3 25915
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 ATP6V1E1 529
Co-fractionation Homo sapiens
70 NDUFV1 4723
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
71 Rab5c 19345
Affinity Capture-MS Mus musculus
72 C15orf48  
Affinity Capture-MS Homo sapiens
73 HADHB 3032
Co-fractionation Homo sapiens
74 HADHA 3030
Co-fractionation Homo sapiens
75 Cd2ap 12488
Affinity Capture-MS Mus musculus
76 NDUFB10 4716
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
77 NDUFA13 51079
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 LRRC59 55379
Co-fractionation Homo sapiens
79 NDUFA9 4704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
80 ND5 4540
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 UQCRC2 7385
Co-fractionation Homo sapiens
82 Bmpr1a  
Affinity Capture-MS Mus musculus
83 GOLT1B 51026
Affinity Capture-MS Homo sapiens
84 ATP1B3 483
Co-fractionation Homo sapiens
85 LOR  
Affinity Capture-MS Homo sapiens
86 CORO1C 23603
Co-fractionation Homo sapiens
87 NDUFA11 126328
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 NDUFB3 4709
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 TOMM5  
Co-fractionation Homo sapiens
90 COX6C 1345
Co-fractionation Homo sapiens
91 NDUFB6 4712
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 ACAD9 28976
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 ACTL6B  
Affinity Capture-MS Homo sapiens
94 NDUFA3  
Affinity Capture-MS Homo sapiens
95 VDAC1 7416
Co-fractionation Homo sapiens
96 SDR9C7  
Affinity Capture-MS Homo sapiens
97 NDUFV2 4729
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 NDUFC2 4718
Affinity Capture-MS Homo sapiens
99 METTL15  
Affinity Capture-MS Homo sapiens
100 SCCPDH 51097
Co-fractionation Homo sapiens
101 HUWE1 10075
Affinity Capture-MS Homo sapiens
102 ND6  
Affinity Capture-MS Homo sapiens
103 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
104 PHB2 11331
Co-fractionation Homo sapiens
105 VDAC3 7419
Co-fractionation Homo sapiens
106 SERPINA12  
Affinity Capture-MS Homo sapiens
107 HAL 3034
Affinity Capture-MS Homo sapiens
108 EIF1AX 1964
Affinity Capture-MS Homo sapiens
109 UQCRH 7388
Co-fractionation Homo sapiens
110 COX7C 1350
Co-fractionation Homo sapiens
111 BCKDHA 593
Co-fractionation Homo sapiens
112 PDHA1 5160
Co-fractionation Homo sapiens
113 Htt  
Affinity Capture-MS Mus musculus
114 PDHB 5162
Co-fractionation Homo sapiens
115 PKP1 5317
Affinity Capture-MS Homo sapiens
116 VDAC2 7417
Co-fractionation Homo sapiens
117 NDUFS8 4728
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 NDUFA2 4695
Affinity Capture-MS Homo sapiens
119 ATP5B 506
Co-fractionation Homo sapiens
120 SLC25A51  
Proximity Label-MS Homo sapiens
121 GSDMA 284110
Affinity Capture-MS Homo sapiens
122 COX7A2 1347
Co-fractionation Homo sapiens
123 C1QBP 708
Proximity Label-MS Homo sapiens
124 NDUFAF4 29078
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 ATP5C1 509
Co-fractionation Homo sapiens
126 RAB5C 5878
Affinity Capture-MS Homo sapiens
127 NDUFB11 54539
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
128 MYC  
Affinity Capture-MS Homo sapiens
129 ND4 4538
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
130 NDUFS6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
131 CPT1A 1374
Co-fractionation Homo sapiens
132 ECSIT 51295
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
133 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
134 NDUFB4 4710
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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