Gene description for CNOT4
Gene name CCR4-NOT transcription complex, subunit 4
Gene symbol CNOT4
Other names/aliases CLONE243
NOT4
NOT4H
Species Homo sapiens
 Database cross references - CNOT4
ExoCarta ExoCarta_4850
Vesiclepedia VP_4850
Entrez Gene 4850
HGNC 7880
MIM 604911
UniProt O95628  
 CNOT4 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for CNOT4
Molecular Function
    RNA binding GO:0003723 HDA
    ubiquitin-protein transferase activity GO:0004842 IBA
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    nuclear-transcribed mRNA poly(A) tail shortening GO:0000289 NAS
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    protein ubiquitination GO:0016567 IBA
    regulation of megakaryocyte differentiation GO:0045652 IDA
    protein autoubiquitination GO:0051865 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 TAS
    CCR4-NOT complex GO:0030014 IBA
    CCR4-NOT complex GO:0030014 IDA
    CCR4-NOT complex GO:0030014 NAS
 Experiment description of studies that identified CNOT4 in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for CNOT4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2W  
Two-hybrid Homo sapiens
2 UBE2N 7334
Two-hybrid Homo sapiens
3 BCAS2 10286
Two-hybrid Homo sapiens
4 XPO1 7514
Affinity Capture-MS Homo sapiens
5 CNOT2  
Co-fractionation Homo sapiens
6 UBE2E3 10477
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 PTEN 5728
Negative Genetic Homo sapiens
8 SQSTM1 8878
Proximity Label-MS Homo sapiens
9 UBE2E1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
11 VHL  
Negative Genetic Homo sapiens
12 RBM15 64783
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
13 UBE2D4  
Two-hybrid Homo sapiens
14 CNOT3 4849
Co-fractionation Homo sapiens
15 PLOD1 5351
Co-fractionation Homo sapiens
16 TNKS1BP1 85456
Co-fractionation Homo sapiens
17 AP2M1 1173
Co-fractionation Homo sapiens
18 CNOT4 4850
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
19 UBE2D2 7322
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
20 CLIC3 9022
Affinity Capture-MS Homo sapiens
21 CNOT1 23019
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
22 YAP1 10413
Affinity Capture-Western Homo sapiens
23 MCM2 4171
Affinity Capture-MS Homo sapiens
24 MICALL1 85377
Co-fractionation Homo sapiens
25 BICD1 636
Proximity Label-MS Homo sapiens
26 UBE2D3 7323
Two-hybrid Homo sapiens
27 CNOT10 25904
Co-fractionation Homo sapiens
28 LMNA 4000
Affinity Capture-MS Homo sapiens
29 KDM5C  
Biochemical Activity Homo sapiens
30 EP300 2033
Two-hybrid Homo sapiens
31 UBE2D1 7321
Reconstituted Complex Homo sapiens
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