Experiment description of studies that identified FAM193B in sEVs

1 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

2 Experiment ID 224 MISEV standards ✔ EM|AFM Biophysical techniques ✔ Alix|TSG101|CD63|CD81 Enriched markers ✔ GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25944692     Organism Homo sapiens Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S" Journal name Oncotarget Publication year 2015 Sample Neuroblastoma cells Sample name SH-SY5Y Isolation/purification methods Differential centrifugation Ultracentrifugation OptiPrep density gradient Flotation density 1.10 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting

Protein-protein interactions for FAM193B

Protein Interactor ExoCarta ID Identification method PubMed Species 1 TRIM10   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 2 LDOC1   Two-hybrid Homo sapiens 3 CDC25A   Two-hybrid Homo sapiens 4 ENO1 2023 Cross-Linking-MS (XL-MS) Homo sapiens 5 S100A2 6273 Affinity Capture-MS Homo sapiens 6 PKN2 5586 Two-hybrid Homo sapiens 7 FBXW11   Affinity Capture-MS Homo sapiens 8 GPR183   Affinity Capture-MS Homo sapiens 9 GSK3A 2931 Two-hybrid Homo sapiens 10 CNOT11 55571 Two-hybrid Homo sapiens 11 SPANXN2   Two-hybrid Homo sapiens 12 MAPK8 5599 Two-hybrid Homo sapiens 13 CUL3 8452 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 14 ELAVL1 1994 Affinity Capture-RNA Homo sapiens 15 TRIM23 373 Two-hybrid Homo sapiens 16 GSK3B 2932 Two-hybrid Homo sapiens 17 TDRKH   Affinity Capture-MS Homo sapiens 18 KCTD5 54442 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 19 TRIM27   Two-hybrid Homo sapiens 20 GOLGA2 2801 Two-hybrid Homo sapiens 21 ATXN1 6310 Two-hybrid Homo sapiens View the network

Pathways in which FAM193B is involved

No pathways found