Gene description for CPSF3L
Gene name cleavage and polyadenylation specific factor 3-like
Gene symbol CPSF3L
Other names/aliases CPSF73L
INT11
INTS11
RC-68
RC68
Species Homo sapiens
 Database cross references - CPSF3L
ExoCarta ExoCarta_54973
Vesiclepedia VP_54973
Entrez Gene 54973
HGNC 26052
MIM 611354
UniProt Q5TA45  
 CPSF3L identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for CPSF3L
Molecular Function
    RNA endonuclease activity GO:0004521 IBA
    RNA endonuclease activity GO:0004521 IMP
    protein binding GO:0005515 IPI
    protein binding GO:0005515 IPI
Biological Process
    snRNA processing GO:0016180 IBA
    snRNA processing GO:0016180 IDA
    snRNA processing GO:0016180 NAS
    regulation of transcription elongation by RNA polymerase II GO:0034243 NAS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    integrator complex GO:0032039 IDA
    integrator complex GO:0032039 NAS
    blood microparticle GO:0072562 HDA
 Experiment description of studies that identified CPSF3L in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CPSF3L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLR2B 5431
Affinity Capture-MS Homo sapiens
2 INTS4  
Co-fractionation Homo sapiens
3 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 PRR35  
Two-hybrid Homo sapiens
6 BAG2 9532
Affinity Capture-MS Homo sapiens
7 TK1 7083
Two-hybrid Homo sapiens
8 KPNA1 3836
Affinity Capture-MS Homo sapiens
9 KRTAP15-1  
Two-hybrid Homo sapiens
10 PLSCR1 5359
Two-hybrid Homo sapiens
11 INTS10  
Affinity Capture-MS Homo sapiens
12 PLSCR4  
Two-hybrid Homo sapiens
13 INTS2  
Affinity Capture-Western Homo sapiens
14 MDFI  
Two-hybrid Homo sapiens
15 TCF7L2  
Two-hybrid Homo sapiens
16 NABP2  
Affinity Capture-MS Homo sapiens
17 C7orf76  
Affinity Capture-MS Homo sapiens
18 ZIC1  
Two-hybrid Homo sapiens
19 INTS1 26173
Affinity Capture-MS Homo sapiens
20 INTS9 55756
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
21 APEX1 328
Affinity Capture-RNA Homo sapiens
22 OXER1  
Two-hybrid Homo sapiens
23 TRIM28 10155
Affinity Capture-MS Homo sapiens
24 POLR2I 5438
Affinity Capture-MS Homo sapiens
25 Ints4  
Affinity Capture-MS Mus musculus
26 TBX6  
Two-hybrid Homo sapiens
27 SNRPC 6631
Two-hybrid Homo sapiens
28 INTS6 26512
Affinity Capture-MS Homo sapiens
29 SPIC  
Two-hybrid Homo sapiens
30 MECP2 4204
Affinity Capture-MS Homo sapiens
31 CSNK2B 1460
Two-hybrid Homo sapiens
32 DHDH  
Affinity Capture-MS Homo sapiens
33 NABP1  
Affinity Capture-MS Homo sapiens
34 POLR2K  
Affinity Capture-MS Homo sapiens
35 ANXA7 310
Two-hybrid Homo sapiens
36 FAM222B  
Two-hybrid Homo sapiens
37 PTPRN2  
Affinity Capture-MS Homo sapiens
38 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
39 TLX3  
Two-hybrid Homo sapiens
40 INTS5 80789
Affinity Capture-MS Homo sapiens
41 SUPT5H 6829
Affinity Capture-MS Homo sapiens
42 Ppp2ca 19052
Affinity Capture-MS Mus musculus
43 NSMF  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
44 INTS7  
Co-fractionation Homo sapiens
45 PLK1 5347
Two-hybrid Homo sapiens
46 PPP2CA 5515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PKN1 5585
Two-hybrid Homo sapiens
48 NME3 4832
Affinity Capture-MS Homo sapiens
49 RPLP1 6176
Affinity Capture-MS Homo sapiens
50 ENY2 56943
Affinity Capture-MS Homo sapiens
51 VENTX  
Two-hybrid Homo sapiens
52 GPS2  
Two-hybrid Homo sapiens
53 LY86  
Affinity Capture-MS Homo sapiens
54 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
55 TRIP13 9319
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 SPAG8  
Two-hybrid Homo sapiens
57 THBS3 7059
Affinity Capture-MS Homo sapiens
58 CCDC120  
Two-hybrid Homo sapiens
59 POLR2E 5434
Affinity Capture-MS Homo sapiens
60 CUL3 8452
Affinity Capture-MS Homo sapiens
61 POLR2A 5430
Affinity Capture-Western Homo sapiens
62 OTX1  
Two-hybrid Homo sapiens
63 TFG 10342
Two-hybrid Homo sapiens
64 ZC3H10  
Two-hybrid Homo sapiens
65 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 RPL35A 6165
Affinity Capture-MS Homo sapiens
67 C1orf94  
Two-hybrid Homo sapiens
68 DSE 29940
Two-hybrid Homo sapiens
69 CBWD1 55871
Affinity Capture-MS Homo sapiens
70 PPP2CB 5516
Affinity Capture-MS Homo sapiens
71 POLR2F  
Affinity Capture-MS Homo sapiens
72 PARP1 142
Proximity Label-MS Homo sapiens
73 PROP1  
Two-hybrid Homo sapiens
74 HSF4  
Two-hybrid Homo sapiens
75 VAC14 55697
Two-hybrid Homo sapiens
76 COMMD5 28991
Affinity Capture-MS Homo sapiens
77 VWA9  
Affinity Capture-MS Homo sapiens
78 LENG8  
Two-hybrid Homo sapiens
79 SHFM1 7979
Affinity Capture-MS Homo sapiens
80 DDX58 23586
Affinity Capture-RNA Homo sapiens
81 RAD9B  
Affinity Capture-MS Homo sapiens
82 CREB5 9586
Two-hybrid Homo sapiens
83 KRTAP26-1  
Two-hybrid Homo sapiens
84 INF2 64423
Affinity Capture-MS Homo sapiens
85 BRD1 23774
Affinity Capture-MS Homo sapiens
86 INTS3 65123
Affinity Capture-MS Homo sapiens
87 KRAS 3845
Negative Genetic Homo sapiens
88 SSRP1 6749
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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