Gene description for FAM63A
Gene name family with sequence similarity 63, member A
Gene symbol FAM63A
Other names/aliases -
Species Homo sapiens
 Database cross references - FAM63A
ExoCarta ExoCarta_55793
Vesiclepedia VP_55793
Entrez Gene 55793
HGNC 25648
UniProt Q8N5J2  
 FAM63A identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for FAM63A
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IEA
    protein binding GO:0005515 IPI
    cysteine-type carboxypeptidase activity GO:0016807 IBA
    cysteine-type carboxypeptidase activity GO:0016807 IDA
    K48-linked polyubiquitin modification-dependent protein binding GO:0036435 IDA
    K48-linked deubiquitinase activity GO:1990380 IBA
    K48-linked deubiquitinase activity GO:1990380 IDA
Biological Process
    proteolysis GO:0006508 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nuclear body GO:0016604 IDA
 Experiment description of studies that identified FAM63A in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FAM63A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SV2C  
Affinity Capture-MS Homo sapiens
2 SEL1L3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 OR6M1  
Affinity Capture-MS Homo sapiens
4 TSPAN16  
Affinity Capture-MS Homo sapiens
5 TMEM63C  
Affinity Capture-MS Homo sapiens
6 BTBD6  
Affinity Capture-MS Homo sapiens
7 CNGA4  
Affinity Capture-MS Homo sapiens
8 LSM8 51691
Two-hybrid Homo sapiens
9 SLC45A2 51151
Affinity Capture-MS Homo sapiens
10 NRSN1  
Affinity Capture-MS Homo sapiens
11 CHRNB1  
Affinity Capture-MS Homo sapiens
12 OR1A2  
Affinity Capture-MS Homo sapiens
13 MANSC1 54682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 OR5H1  
Affinity Capture-MS Homo sapiens
15 CD1D 912
Affinity Capture-MS Homo sapiens
16 UBC 7316
Reconstituted Complex Homo sapiens
17 CHIA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 FBXW12  
Affinity Capture-MS Homo sapiens
19 OR10S1  
Affinity Capture-MS Homo sapiens
20 OR2G6  
Affinity Capture-MS Homo sapiens
21 RNF126  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ZP3 7784
Affinity Capture-MS Homo sapiens
23 PTCHD3  
Affinity Capture-MS Homo sapiens
24 KDELR1 10945
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 DAZAP2  
Two-hybrid Homo sapiens
26 YAP1 10413
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 KCNA10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 GRTP1 79774
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 TMEM74  
Affinity Capture-MS Homo sapiens
30 TMEM31  
Affinity Capture-MS Homo sapiens
31 GMCL1  
Two-hybrid Homo sapiens
32 DDX58 23586
Affinity Capture-RNA Homo sapiens
33 PIGH  
Affinity Capture-MS Homo sapiens
34 HERC2 8924
Affinity Capture-MS Homo sapiens
35 RGS9BP  
Affinity Capture-MS Homo sapiens
36 CTLA4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which FAM63A is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here